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TitleAn improved fluorescent tag and its nanobodies for membrane protein expression, stability assay, and purification.
Journal, issue, pagesCommun Biol, Vol. 3, Page 753-753, Year 2020
Publish dateFeb 17, 2020 (structure data deposition date)
AuthorsCai, H. / Yao, H. / Li, T. / Hutter, C.A.J. / Li, Y. / Tang, Y. / Seeger, M.A. / Li, D.
External linksCommun Biol / PubMed:33303987
MethodsX-ray diffraction
Resolution2.03 Å
Structure data

PDB-6lz2:
Crystal structure of a thermostable green fluorescent protein (TGP) with a synthetic nanobody (Sb44)
Method: X-RAY DIFFRACTION / Resolution: 2.03 Å

Chemicals

ChemComp-NA:
Unknown entry

ChemComp-ACT:
ACETATE ION

ChemComp-GOL:
GLYCEROL

ChemComp-TRS:
2-AMINO-2-HYDROXYMETHYL-PROPANE-1,3-DIOL / pH buffer*YM

ChemComp-HOH:
WATER

Source
  • galaxea fascicularis (invertebrata)
  • lama glama (llama)
KeywordsFLUORESCENT PROTEIN / complex / GFP / nanobody / single-chain antibody / sybody / synthetic antibody / TGP / thermostable green fluorescent protein

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