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TitleStructural insight into the assembly and conformational activation of human origin recognition complex.
Journal, issue, pagesCell Discov, Vol. 6, Issue 1, Page 88, Year 2020
Publish dateNov 24, 2020
AuthorsJiaxuan Cheng / Ningning Li / Xiaohan Wang / Jiazhi Hu / Yuanliang Zhai / Ning Gao /
PubMed AbstractThe function of the origin recognition complex (ORC) in DNA replication is highly conserved in recognizing and marking the initiation sites. The detailed molecular mechanisms by which human ORC is ...The function of the origin recognition complex (ORC) in DNA replication is highly conserved in recognizing and marking the initiation sites. The detailed molecular mechanisms by which human ORC is reconfigured into a state competent for origin association remain largely unknown. Here, we present structural characterizations of human ORC1-5 and ORC2-5 assemblies. ORC2-5 exhibits a tightly autoinhibited conformation with the winged-helix domain of ORC2 completely blocking the central DNA-binding channel. The binding of ORC1 partially relieves the autoinhibitory effect of ORC2-5 through remodeling ORC2-WHD, which makes ORC2-WHD away from the central channel creating a still autoinhibited but more dynamic structure. In particular, the AAA+ domain of ORC1 is highly flexible to sample a variety of conformations from inactive to potentially active states. These results provide insights into the detailed mechanisms regulating the autoinhibition of human ORC and its subsequent activation for DNA binding.
External linksCell Discov / PubMed:33298899 / PubMed Central
MethodsEM (single particle)
Resolution3.8 - 5.0 Å
Structure data

30462

7cte

EMDB-30462, PDB-7cte:
Human Origin Recognition Complex, ORC2-5
Method: EM (single particle) / Resolution: 3.8 Å

30463

7ctf

EMDB-30463, PDB-7ctf:
Human origin recognition complex 1-5 State II
Method: EM (single particle) / Resolution: 4.8 Å

30464

7ctg

EMDB-30464, PDB-7ctg:
Human Origin Recognition Complex, ORC1-5 State I
Method: EM (single particle) / Resolution: 5.0 Å

EMDB-30467:
HsORC2-5 with the local density of ORC2-WHD improved
Method: EM (single particle) / Resolution: 3.9 Å

Chemicals

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

Source
  • homo sapiens (human)
KeywordsREPLICATION / DNA replication initiation / Origin Recognition Complex (ORC) / cryo-EM / autoinhibition conformation / Origin Recognition Complex

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