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TitleCalcium depletion challenges endoplasmic reticulum proteostasis by destabilising BiP-substrate complexes.
Journal, issue, pagesElife, Vol. 9, Year 2020
Publish dateAug 1, 2020 (structure data deposition date)
AuthorsPreissler, S. / Rato, C. / Yan, Y. / Perera, L.A. / Czako, A. / Ron, D.
External linksElife / PubMed:33295873
MethodsX-ray diffraction
Resolution1.52 - 1.94 Å
Structure data

PDB-6zyh:
Crystal structure of GRP78 (70kDa heat shock protein 5 / BiP) ATPase domain in complex with ADP and calcium
Method: X-RAY DIFFRACTION / Resolution: 1.88 Å

PDB-6zyi:
Crystal structure of HSP70 ATPase domain in complex with ADP and calcium
Method: X-RAY DIFFRACTION / Resolution: 1.52 Å

PDB-6zyj:
Crystal structure of Hsc70 ATPase domain in complex with ADP and calcium
Method: X-RAY DIFFRACTION / Resolution: 1.85 Å

PDB-7a4u:
Crystal structure of lid-truncated apo BiP in an oligomeric state
Method: X-RAY DIFFRACTION / Resolution: 1.77 Å

PDB-7a4v:
Crystal structure of lid-truncated ADP-bound BiP in an oligomeric state
Method: X-RAY DIFFRACTION / Resolution: 1.94 Å

Chemicals

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

ChemComp-CA:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-ACT:
ACETATE ION

ChemComp-GOL:
GLYCEROL

ChemComp-K:
Unknown entry

Source
  • cricetulus griseus (Chinese hamster)
  • homo sapiens (human)
KeywordsCHAPERONE / BiP / HSP70 / GRP78 / ATPase domain / NBD / ADP / Hsc70 / ER / Endoplasmic reticulum

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