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Structure paper

TitleMolecular basis of regio- and stereo-specificity in biosynthesis of bacterial heterodimeric diketopiperazines.
Journal, issue, pagesNat Commun, Vol. 11, Page 6251-6251, Year 2020
Publish dateFeb 24, 2020 (structure data deposition date)
AuthorsSun, C. / Luo, Z. / Zhang, W. / Tian, W. / Peng, H. / Lin, Z. / Deng, Z. / Kobe, B. / Jia, X. / Qu, X.
External linksNat Commun / PubMed:33288748
MethodsX-ray diffraction
Resolution1.47 - 1.7 Å
Structure data

PDB-6vxv:
Crystal structure of cyclo-L-Trp-L-Pro-bound cytochrome P450 NasF5053 from Streptomyces sp. NRRL F-5053
Method: X-RAY DIFFRACTION / Resolution: 1.6 Å

PDB-6vza:
Crystal structure of cytochrome P450 NasF5053 Q65I-A86G mutant variant from Streptomyces sp. NRRL F-5053 in the cyclo-L-Trp-L-Pro-bound state
Method: X-RAY DIFFRACTION / Resolution: 1.47 Å

PDB-6vzb:
Crystal structure of cytochrome P450 NasF5053 S284A-V288A mutant variant from Streptomyces sp. NRRL F-5053 in the cyclo-L-Trp-L-Pro-bound state
Method: X-RAY DIFFRACTION / Resolution: 1.68 Å

PDB-6w0s:
Crystal structure of substrate free cytochrome P450 NasF5053 from Streptomyces sp. NRRL F-5053
Method: X-RAY DIFFRACTION / Resolution: 1.7 Å

Chemicals

ChemComp-NA:
Unknown entry

ChemComp-QRP:
(3S,8aS)-3-(1H-indol-3-ylmethyl)hexahydropyrrolo[1,2-a]pyrazine-1,4-dione / antifungal*YM

ChemComp-HEM:
PROTOPORPHYRIN IX CONTAINING FE

ChemComp-CA:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-CL:
Unknown entry

ChemComp-GOL:
GLYCEROL

ChemComp-BR:
BROMIDE ION

Source
  • streptomyces sp. nrrl f-5053 (bacteria)
KeywordsOXIDOREDUCTASE / biosynthetic protein / cytochrome P450 / cyclodipeptide / regio-selecitivty / stereo-selectivity / pyrroloindoline alkaloids / radical mediated reaction / F5053 / Streptomyces

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