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TitleStructure of the shutdown state of myosin-2.
Journal, issue, pagesNature, Vol. 588, Issue 7838, Page 515-520, Year 2020
Publish dateDec 2, 2020
AuthorsCharlotte A Scarff / Glenn Carrington / David Casas-Mao / Joseph M Chalovich / Peter J Knight / Neil A Ranson / Michelle Peckham /
PubMed AbstractMyosin-2 is essential for processes as diverse as cell division and muscle contraction. Dephosphorylation of its regulatory light chain promotes an inactive, 'shutdown' state with the filament- ...Myosin-2 is essential for processes as diverse as cell division and muscle contraction. Dephosphorylation of its regulatory light chain promotes an inactive, 'shutdown' state with the filament-forming tail folded onto the two heads, which prevents filament formation and inactivates the motors. The mechanism by which this happens is unclear. Here we report a cryo-electron microscopy structure of shutdown smooth muscle myosin with a resolution of 6 Å in the head region. A pseudo-atomic model, obtained by flexible fitting of crystal structures into the density and molecular dynamics simulations, describes interaction interfaces at the atomic level. The N-terminal extension of one regulatory light chain interacts with the tail, and the other with the partner head, revealing how the regulatory light chains stabilize the shutdown state in different ways and how their phosphorylation would allow myosin activation. Additional interactions between the three segments of the coiled coil, the motor domains and the light chains stabilize the shutdown molecule. The structure of the lever in each head is competent to generate force upon activation. This shutdown structure is relevant to all isoforms of myosin-2 and provides a framework for understanding their disease-causing mutations.
External linksNature / PubMed:33268888 / PubMed Central
MethodsEM (single particle)
Resolution6.3 - 9.0 Å
Structure data

11069

6z47

EMDB-11069, PDB-6z47:
Smooth muscle myosin shutdown state heads region
Method: EM (single particle) / Resolution: 6.3 Å

EMDB-11070:
Smooth muscle myosin shutdown state
Method: EM (single particle) / Resolution: 9.0 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM / Adenosine diphosphate

ChemComp-PO4:
PHOSPHATE ION / Phosphate

Source
  • meleagris gallopavo (turkey)
  • turkey (turkey)
KeywordsCONTRACTILE PROTEIN / myosin / motor / inhibited state / shutdown state / smooth muscle

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