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TitleDistinct Structures and Dynamics of Chromatosomes with Different Human Linker Histone Isoforms.
Journal, issue, pagesMol Cell, Vol. 81, Issue 1, Page 166-182.e6, Year 2021
Publish dateJan 7, 2021
AuthorsBing-Rui Zhou / Hanqiao Feng / Seyit Kale / Tara Fox / Htet Khant / Natalia de Val / Rodolfo Ghirlando / Anna R Panchenko / Yawen Bai /
PubMed AbstractThe repeating structural unit of metazoan chromatin is the chromatosome, a nucleosome bound to a linker histone, H1. There are 11 human H1 isoforms with diverse cellular functions, but how they ...The repeating structural unit of metazoan chromatin is the chromatosome, a nucleosome bound to a linker histone, H1. There are 11 human H1 isoforms with diverse cellular functions, but how they interact with the nucleosome remains elusive. Here, we determined the cryoelectron microscopy (cryo-EM) structures of chromatosomes containing 197 bp DNA and three different human H1 isoforms, respectively. The globular domains of all three H1 isoforms bound to the nucleosome dyad. However, the flanking/linker DNAs displayed substantial distinct dynamic conformations. Nuclear magnetic resonance (NMR) and H1 tail-swapping cryo-EM experiments revealed that the C-terminal tails of the H1 isoforms mainly controlled the flanking DNA orientations. We also observed partial ordering of the core histone H2A C-terminal and H3 N-terminal tails in the chromatosomes. Our results provide insights into the structures and dynamics of the chromatosomes and have implications for the structure and function of chromatin.
External linksMol Cell / PubMed:33238161 / PubMed Central
MethodsEM (single particle)
Resolution2.76 - 9.73 Å
Structure data

22683

7k5x

EMDB-22683, PDB-7k5x:
Cryo-EM structure of a chromatosome containing human linker histone H1.0
Method: EM (single particle) / Resolution: 2.93 Å

22684

7k5y

EMDB-22684, PDB-7k5y:
Cryo-EM structure of a chromatosome containing human linker histone H1.4
Method: EM (single particle) / Resolution: 2.76 Å

22685

7k60

EMDB-22685, PDB-7k60:
Cryo-EM structure of a chromatosome containing human linker histone H1.10
Method: EM (single particle) / Resolution: 3.12 Å

22686

7k61

EMDB-22686, PDB-7k61:
Cryo-EM structure of 197bp nucleosome aided by scFv
Method: EM (single particle) / Resolution: 2.85 Å

22687

7k63

EMDB-22687, PDB-7k63:
Cryo-EM structure of a chromatosome containing chimeric linker histone gH1.10-ncH1.4
Method: EM (single particle) / Resolution: 3.03 Å

EMDB-22688:
cryo-EM map of di-chromatosome containing H1.4
Method: EM (single particle) / Resolution: 9.73 Å

Source
  • human (human)
  • homo sapiens (human)
  • mus musculus (house mouse)
  • Mougeotia scalaris (plant)
KeywordsNUCLEAR PROTEIN/DNA / chromatosome / nucleosome / linker histones / Single-chain antibody / charge-charge interaction / chromatin / NUCLEAR PROTEIN / NUCLEAR PROTEIN-DNA complex

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