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TitleCrystal structures of gamma-glutamylmethylamide synthetase provide insight into bacterial metabolism of oceanic monomethylamine.
Journal, issue, pagesJ. Biol. Chem., Vol. 296, Page 100081-100081, Year 2020
Publish dateAug 11, 2020 (structure data deposition date)
AuthorsWang, N. / Chen, X.L. / Gao, C. / Peng, M. / Wang, P. / Zhang, N. / Li, F. / Yang, G.P. / Shen, Q.T. / Li, S. ...Wang, N. / Chen, X.L. / Gao, C. / Peng, M. / Wang, P. / Zhang, N. / Li, F. / Yang, G.P. / Shen, Q.T. / Li, S. / Chen, Y. / Zhang, Y.Z. / Li, C.Y.
External linksJ. Biol. Chem. / PubMed:33199371
MethodsX-ray diffraction
Resolution1.962 - 2.801 Å
Structure data

PDB-7cql:
Apo GmaS without ligand
Method: X-RAY DIFFRACTION / Resolution: 2.801 Å

PDB-7cqn:
GmaS in complex with AMPPCP
Method: X-RAY DIFFRACTION / Resolution: 1.962 Å

PDB-7cqq:
GmaS in complex with AMPPNP and MetSox
Method: X-RAY DIFFRACTION / Resolution: 2.295 Å

PDB-7cqu:
GmaS/ADP/MetSox-P complex
Method: X-RAY DIFFRACTION / Resolution: 2.06 Å

PDB-7cqw:
GmaS/ADP complex-Conformation 1
Method: X-RAY DIFFRACTION / Resolution: 2.297 Å

PDB-7cqx:
GmaS/ADP complex-Conformation 2
Method: X-RAY DIFFRACTION / Resolution: 2.301 Å

Chemicals

ChemComp-ACP:
PHOSPHOMETHYLPHOSPHONIC ACID ADENYLATE ESTER / AMP-PCP, energy-carrying molecule analogue*YM

ChemComp-HOH:
WATER

ChemComp-MG:
Unknown entry

ChemComp-ANP:
PHOSPHOAMINOPHOSPHONIC ACID-ADENYLATE ESTER / AMP-PNP, energy-carrying molecule analogue*YM

ChemComp-MSL:
(2S)-2-AMINO-4-(METHYLSULFONIMIDOYL)BUTANOIC ACID / inhibitor*YM

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

ChemComp-P3S:
L-METHIONINE-S-SULFOXIMINE PHOSPHATE

Source
  • rhodovulum sp. 12e13 (bacteria)
KeywordsLIGASE / GMA synthetase / BIOSYNTHETIC PROTEIN

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