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Structure paper

TitleA non-helical region in transmembrane helix 6 of hydrophobic amino acid transporter MhsT mediates substrate recognition.
Journal, issue, pagesEmbo J., Vol. 40, Page e105164-e105164, Year 2021
Publish dateApr 25, 2020 (structure data deposition date)
AuthorsFocht, D. / Neumann, C. / Lyons, J. / Eguskiza Bilbao, A. / Blunck, R. / Malinauskaite, L. / Schwarz, I.O. / Javitch, J.A. / Quick, M. / Nissen, P.
External linksEmbo J. / PubMed:33155685
MethodsX-ray diffraction
Resolution2.25 - 3.1 Å
Structure data

PDB-6yu2:
Crystal structure of MhsT in complex with L-isoleucine
Method: X-RAY DIFFRACTION / Resolution: 3.1 Å

PDB-6yu3:
Crystal structure of MhsT in complex with L-phenylalanine
Method: X-RAY DIFFRACTION / Resolution: 2.25 Å

PDB-6yu4:
Crystal structure of MhsT in complex with L-4F-phenylalanine
Method: X-RAY DIFFRACTION / Resolution: 2.26 Å

PDB-6yu5:
Crystal structure of MhsT in complex with L-valine
Method: X-RAY DIFFRACTION / Resolution: 2.6 Å

PDB-6yu6:
Crystal structure of MhsT in complex with L-leucine
Method: X-RAY DIFFRACTION / Resolution: 2.35 Å

PDB-6yu7:
Crystal structure of MhsT in complex with L-tyrosine
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

Chemicals

ChemComp-BOG:
octyl beta-D-glucopyranoside / detergent*YM

ChemComp-NA:
Unknown entry

ChemComp-ILE:
ISOLEUCINE

ChemComp-HOH:
WATER

ChemComp-PHE:
PHENYLALANINE

ChemComp-LMT:
DODECYL-BETA-D-MALTOSIDE / detergent*YM

ChemComp-SOG:
octyl 1-thio-beta-D-glucopyranoside / detergent*YM

ChemComp-GOL:
GLYCEROL

ChemComp-PFF:
4-FLUORO-L-PHENYLALANINE

ChemComp-PCW:
1,2-DIOLEOYL-SN-GLYCERO-3-PHOSPHOCHOLINE / DOPC, phospholipid*YM

ChemComp-VAL:
VALINE

ChemComp-BNG:
nonyl beta-D-glucopyranoside / detergent*YM

ChemComp-LEU:
LEUCINE

ChemComp-TYR:
TYROSINE

Source
  • bacillus halodurans (bacteria)
KeywordsTRANSPORT PROTEIN / LeuT-fold / amino acid transporter / L-isoleucine / L-phenylalanine / L-4F-phenylalanine / L-valine / L-leucine / L-tyrosine

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