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TitleInsights into Lysosomal PI(3,5)P Homeostasis from a Structural-Biochemical Analysis of the PIKfyve Lipid Kinase Complex.
Journal, issue, pagesMol Cell, Vol. 80, Issue 4, Page 736-743.e4, Year 2020
Publish dateNov 19, 2020
AuthorsJoshua A Lees / PeiQi Li / Nikit Kumar / Lois S Weisman / Karin M Reinisch /
PubMed AbstractThe phosphoinositide PI(3,5)P, generated exclusively by the PIKfyve lipid kinase complex, is key for lysosomal biology. Here, we explore how PI(3,5)P levels within cells are regulated. We find the ...The phosphoinositide PI(3,5)P, generated exclusively by the PIKfyve lipid kinase complex, is key for lysosomal biology. Here, we explore how PI(3,5)P levels within cells are regulated. We find the PIKfyve complex comprises five copies of the scaffolding protein Vac14 and one copy each of the lipid kinase PIKfyve, generating PI(3,5)P from PI3P and the lipid phosphatase Fig4, reversing the reaction. Fig4 is active as a lipid phosphatase in the ternary complex, whereas PIKfyve within the complex cannot access membrane-incorporated phosphoinositides due to steric constraints. We find further that the phosphoinositide-directed activities of both PIKfyve and Fig4 are regulated by protein-directed activities within the complex. PIKfyve autophosphorylation represses its lipid kinase activity and stimulates Fig4 lipid phosphatase activity. Further, Fig4 is also a protein phosphatase acting on PIKfyve to stimulate its lipid kinase activity, explaining why catalytically active Fig4 is required for maximal PI(3,5)P production by PIKfyve in vivo.
External linksMol Cell / PubMed:33098764 / PubMed Central
MethodsEM (single particle)
Resolution5.1 - 6.6 Å
Structure data

22631

7k1w

EMDB-22631, PDB-7k1w:
PIKfyve/Fig4/Vac14 complex centered on Fig4 - map3
Method: EM (single particle) / Resolution: 5.1 Å

22634

7k1y

EMDB-22634, PDB-7k1y:
PIKfyve/Fig4/Vac14 complex centered on Vac14 - map1
Method: EM (single particle) / Resolution: 5.25 Å

22647

7k2v

EMDB-22647, PDB-7k2v:
PIKfyve/Fig4/Vac14 complex centered on PIKfyve - map2
Method: EM (single particle) / Resolution: 6.6 Å

Source
  • homo sapiens (human)
KeywordsLIPID BINDING PROTEIN / Lipid kinase / Lipid phosphatase / protein complex

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