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Structure paper

TitleSpecific features and assembly of the plant mitochondrial complex I revealed by cryo-EM.
Journal, issue, pagesNat Commun, Vol. 11, Issue 1, Page 5195, Year 2020
Publish dateOct 15, 2020
AuthorsHeddy Soufari / Camila Parrot / Lauriane Kuhn / Florent Waltz / Yaser Hashem /
PubMed AbstractMitochondria are the powerhouses of eukaryotic cells and the site of essential metabolic reactions. Complex I or NADH:ubiquinone oxidoreductase is the main entry site for electrons into the ...Mitochondria are the powerhouses of eukaryotic cells and the site of essential metabolic reactions. Complex I or NADH:ubiquinone oxidoreductase is the main entry site for electrons into the mitochondrial respiratory chain and constitutes the largest of the respiratory complexes. Its structure and composition vary across eukaryote species. However, high resolution structures are available only for one group of eukaryotes, opisthokonts. In plants, only biochemical studies were carried out, already hinting at the peculiar composition of complex I in the green lineage. Here, we report several cryo-electron microscopy structures of the plant mitochondrial complex I. We describe the structure and composition of the plant respiratory complex I, including the ancestral mitochondrial domain composed of the carbonic anhydrase. We show that the carbonic anhydrase is a heterotrimeric complex with only one conserved active site. This domain is crucial for the overall stability of complex I as well as a peculiar lipid complex composed of cardiolipin and phosphatidylinositols. Moreover, we also describe the structure of one of the plant-specific complex I assembly intermediates, lacking the whole P module, in presence of the maturation factor GLDH. GLDH prevents the binding of the plant specific P1 protein, responsible for the linkage of the P to the P module.
External linksNat Commun / PubMed:33060577 / PubMed Central
MethodsEM (single particle)
Resolution3.47 - 3.8 Å
Structure data

EMDB-11513:
Focused reconstruction of the plant mitochondrial complex I
Method: EM (single particle) / Resolution: 3.47 Å

EMDB-11614, PDB-7a23:
Plant mitochondrial respiratory complex I
Method: EM (single particle) / Resolution: 3.7 Å

EMDB-11615, PDB-7a24:
Assembly intermediate of the plant mitochondrial complex I
Method: EM (single particle) / Resolution: 3.8 Å

Chemicals

ChemComp-SF4:
IRON/SULFUR CLUSTER / Iron–sulfur cluster

ChemComp-FMN:
FLAVIN MONONUCLEOTIDE / Flavin mononucleotide

ChemComp-T7X:
Phosphatidylinositol / Phosphatidylinositol

ChemComp-CDL:
CARDIOLIPIN / phospholipid*YM / Cardiolipin

ChemComp-U10:
UBIQUINONE-10 / Coenzyme Q10

ChemComp-PEV:
(1S)-2-{[(2-AMINOETHOXY)(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL STEARATE / POPE, phospholipid*YM / Phosphatidylethanolamine

ChemComp-FES:
FE2/S2 (INORGANIC) CLUSTER / Iron–sulfur cluster

ChemComp-NDP:
NADPH DIHYDRO-NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE / Nicotinamide adenine dinucleotide phosphate

ChemComp-ZN:
ZINC ION / Zinc

ChemComp-BCT:
BICARBONATE ION / pH buffer*YM / Bicarbonate

Source
  • brassica oleracea (wild cabbage)
  • Wild cabbage (wild cabbage)
KeywordsArabidopsis / Brassica / Carbonic Anhydrases / Cardiolipins / Cryoelectron Microscopy / Electron Transport Complex I / Gene Expression Regulation, Plant / Mitochondria / Mitochondrial Membranes / Plant Proteins / Protein Conformation / Proteomics / MEMBRANE PROTEIN / Respiration / Complex I / plant

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