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Structure paper

TitleHigh-resolution structures of the SARS-CoV-2 2'- O -methyltransferase reveal strategies for structure-based inhibitor design.
Journal, issue, pagesSci. Signal., Vol. 13, Year 2020
Publish dateMar 10, 2020 (structure data deposition date)
AuthorsRosas-Lemus, M. / Minasov, G. / Shuvalova, L. / Inniss, N.L. / Kiryukhina, O. / Brunzelle, J. / Satchell, K.J.F.
External linksSci. Signal. / PubMed:32994211
MethodsX-ray diffraction
Resolution1.8 - 2.25 Å
Structure data

PDB-6w4h:
1.80 Angstrom Resolution Crystal Structure of NSP16 - NSP10 Complex from SARS-CoV-2
Method: X-RAY DIFFRACTION / Resolution: 1.8 Å

PDB-6w75:
1.95 Angstrom Resolution Crystal Structure of NSP10 - NSP16 Complex from SARS-CoV-2
Method: X-RAY DIFFRACTION / Resolution: 1.951 Å

PDB-6wjt:
2.0 Angstrom Resolution Crystal Structure of Nsp16-Nsp10 Heterodimer from SARS-CoV-2 in Complex with S-Adenosyl-L-Homocysteine
Method: X-RAY DIFFRACTION / Resolution: 2 Å

PDB-6wkq:
1.98 Angstrom Resolution Crystal Structure of NSP16-NSP10 Heterodimer from SARS-CoV-2 in Complex with Sinefungin
Method: X-RAY DIFFRACTION / Resolution: 1.98 Å

PDB-6wq3:
Crystal Structure of Nsp16-Nsp10 Heterodimer from SARS-CoV-2 in Complex with 7-methyl-GpppA and S-adenosyl-L-homocysteine.
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-6wrz:
Crystal Structure of Nsp16-Nsp10 Heterodimer from SARS-CoV-2 with 7-methyl-GpppA and S-adenosyl-L-homocysteine in the Active Site and Sulfates in the mRNA Binding Groove.
Method: X-RAY DIFFRACTION / Resolution: 2.25 Å

PDB-6wvn:
Crystal Structure of Nsp16-Nsp10 from SARS-CoV-2 in Complex with 7-methyl-GpppA and S-Adenosylmethionine.
Method: X-RAY DIFFRACTION / Resolution: 2 Å

Chemicals

ChemComp-SO3:
SULFITE ION

ChemComp-SAM:
S-ADENOSYLMETHIONINE

ChemComp-ACT:
ACETATE ION

ChemComp-BDF:
beta-D-fructopyranose

ChemComp-ZN:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-NA:
Unknown entry

ChemComp-FMT:
FORMIC ACID

ChemComp-SAH:
S-ADENOSYL-L-HOMOCYSTEINE

ChemComp-SFG:
SINEFUNGIN

ChemComp-GTA:
P1-7-METHYLGUANOSINE-P3-ADENOSINE-5',5'-TRIPHOSPHATE

ChemComp-M7G:
7N-METHYL-8-HYDROGUANOSINE-5'-DIPHOSPHATE

ChemComp-SO4:
SULFATE ION

ChemComp-MGP:
7-METHYL-GUANOSINE-5'-TRIPHOSPHATE

ChemComp-CL:
Unknown entry

ChemComp-ADE:
ADENINE

Source
  • severe acute respiratory syndrome coronavirus 2
KeywordsVIRAL PROTEIN / Structural Genomics / Center for Structural Genomics of Infectious Diseases / CSGID / nsp16 / nsp10 / complex / S-Adenosyl-L-Homocysteine / 7-methyl-GpppA

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