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TitleThe molecular structure of mammalian primary cilia revealed by cryo-electron tomography.
Journal, issue, pagesNat Struct Mol Biol, Vol. 27, Issue 12, Page 1115-1124, Year 2020
Publish dateSep 28, 2020
AuthorsPetra Kiesel / Gonzalo Alvarez Viar / Nikolai Tsoy / Riccardo Maraspini / Peter Gorilak / Vladimir Varga / Alf Honigmann / Gaia Pigino /
PubMed AbstractPrimary cilia are microtubule-based organelles that are important for signaling and sensing in eukaryotic cells. Unlike the thoroughly studied motile cilia, the three-dimensional architecture and ...Primary cilia are microtubule-based organelles that are important for signaling and sensing in eukaryotic cells. Unlike the thoroughly studied motile cilia, the three-dimensional architecture and molecular composition of primary cilia are largely unexplored. Yet, studying these aspects is necessary to understand how primary cilia function in health and disease. We developed an enabling method for investigating the structure of primary cilia isolated from MDCK-II cells at molecular resolution by cryo-electron tomography. We show that the textbook '9 + 0' arrangement of microtubule doublets is only present at the primary cilium base. A few microns out, the architecture changes into an unstructured bundle of EB1-decorated microtubules and actin filaments, putting an end to a long debate on the presence or absence of actin filaments in primary cilia. Our work provides a plethora of insights into the molecular structure of primary cilia and offers a methodological framework to study these important organelles.
External linksNat Struct Mol Biol / PubMed:32989303 / PubMed Central
MethodsEM (subtomogram averaging)
Resolution18.56 - 45.0 Å
Structure data

EMDB-10896:
First cryo-ET / subtomogram averaging of primary cilia microtubules show that A-tubules are decorated by EB1
Method: EM (subtomogram averaging) / Resolution: 18.56 Å

EMDB-10900:
cryo-ET/ subtomogram averaging of primary cilia reveals actin filaments in the axoneme
Method: EM (subtomogram averaging) / Resolution: 45.0 Å

Source
  • Canis lupus familiaris (dog)

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