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-Structure paper
Title | Light-coupled cryo-plunger for time-resolved cryo-EM. |
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Journal, issue, pages | J Struct Biol, Vol. 212, Issue 3, Page 107624, Year 2020 |
Publish date | Dec 1, 2020 |
Authors | Nate Yoder / Farzad Jalali-Yazdi / Sigrid Noreng / Alexandra Houser / Isabelle Baconguis / Eric Gouaux / |
PubMed Abstract | Proteins are dynamic molecules that can undergo rapid conformational rearrangements in response to stimuli. These structural changes are often critical to protein function, and thus elucidating time- ...Proteins are dynamic molecules that can undergo rapid conformational rearrangements in response to stimuli. These structural changes are often critical to protein function, and thus elucidating time-dependent conformational landscapes has been a long-standing goal of structural biology. To harness the power of single particle cryo-EM methods to enable 'time-resolved' structure determination, we have developed a light-coupled cryo-plunger that pairs flash-photolysis of caged ligands with rapid sample vitrification. The 'flash-plunger' consists of a high-power ultraviolet LED coupled with focusing optics and a motorized linear actuator, enabling the user to immobilize protein targets in vitreous ice within a programmable time window - as short as tens of milliseconds - after stimulus delivery. The flash-plunger is a simple, inexpensive and flexible tool to explore short-lived conformational states previously unobtainable by conventional sample preparation methods. |
External links | J Struct Biol / PubMed:32950604 / PubMed Central |
Methods | EM (single particle) |
Resolution | 3.5 - 4.0 Å |
Structure data | EMDB-21384: EMDB-21385: |
Source |
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