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Structure paper

TitleStructural Evidence for a [4Fe-5S] Intermediate in the Non-Redox Desulfuration of Thiouracil.
Journal, issue, pagesAngew. Chem. Int. Ed. Engl., Vol. 60, Page 424-431, Year 2021
Publish dateJun 3, 2020 (structure data deposition date)
AuthorsZhou, J. / Pecqueur, L. / Aucynaite, A. / Fuchs, J. / Rutkiene, R. / Vaitekunas, J. / Meskys, R. / Boll, M. / Fontecave, M. / Urbonavicius, J. / Golinelli-Pimpaneau, B.
External linksAngew. Chem. Int. Ed. Engl. / PubMed:32929873
MethodsX-ray diffraction
Resolution1.327 - 2.067 Å
Structure data

PDB-6z92:
[4Fe-4S]-dependent thiouracil desulfidase TudS (DUF523Vcz) solved by Fe-SAD phasing
Method: X-RAY DIFFRACTION / Resolution: 2.067 Å

PDB-6z93:
[4Fe-4S]-dependent thiouracil desulfidase TudS (DUF523Vcz)
Method: X-RAY DIFFRACTION / Resolution: 1.505 Å

PDB-6z94:
[4Fe-4S]-dependent thiouracil desulfidase TudS (DUF523Vcz)(S-SAD data)
Method: X-RAY DIFFRACTION / Resolution: 1.759 Å

PDB-6z96:
[4Fe-4S]-dependent thiouracil desulfidase TudS (DUF523Vcz) soaked with 4-thiouracil (12.65 keV, 7.125 keV (Fe-SAD) and 6.5 keV (S-SAD) data)
Method: X-RAY DIFFRACTION / Resolution: 1.327 Å

PDB-6zw9:
[4Fe-4S]-dependent thiouracil desulfidase TudS (DUF523Vcz) soaked with 4-thiouracil (S-SAD data)
Method: X-RAY DIFFRACTION / Resolution: 1.759 Å

Chemicals

ChemComp-SF4:
IRON/SULFUR CLUSTER

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

ChemComp-HOH:
WATER

ChemComp-CL:
Unknown entry

ChemComp-PGE:
TRIETHYLENE GLYCOL

ChemComp-H2S:
HYDROSULFURIC ACID

Source
  • uncultured bacterium (environmental samples)
KeywordsTRANSFERASE / thiouracil / iron-sulfur cluster / desulfuration / sulfurtransferase

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