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-Structure paper
タイトル | Structures of the stator complex that drives rotation of the bacterial flagellum. |
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ジャーナル・号・ページ | Nat Microbiol, Vol. 5, Issue 12, Page 1553-1564, Year 2020 |
掲載日 | 2020年9月14日 |
著者 | Justin C Deme / Steven Johnson / Owen Vickery / Amy Aron / Holly Monkhouse / Thomas Griffiths / Rory Hennell James / Ben C Berks / James W Coulton / Phillip J Stansfeld / Susan M Lea / |
PubMed 要旨 | The bacterial flagellum is the prototypical protein nanomachine and comprises a rotating helical propeller attached to a membrane-embedded motor complex. The motor consists of a central rotor ...The bacterial flagellum is the prototypical protein nanomachine and comprises a rotating helical propeller attached to a membrane-embedded motor complex. The motor consists of a central rotor surrounded by stator units that couple ion flow across the cytoplasmic membrane to generate torque. Here, we present the structures of the stator complexes from Clostridium sporogenes, Bacillus subtilis and Vibrio mimicus, allowing interpretation of the extensive body of data on stator mechanism. The structures reveal an unexpected asymmetric AB subunit assembly where the five A subunits enclose the two B subunits. Comparison to structures of other ion-driven motors indicates that this AB architecture is fundamental to bacterial systems that couple energy from ion flow to generate mechanical work at a distance and suggests that such events involve rotation in the motor structures. |
リンク | Nat Microbiol / PubMed:32929189 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 3.4 - 4.6 Å |
構造データ | EMDB-10895, PDB-6ysf: EMDB-10897: EMDB-10899, PDB-6ysl: EMDB-10901: EMDB-10902: |
由来 |
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キーワード | MOTOR PROTEIN / Stator Flagellar rotation ion driven motor |