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Structure paper

TitleCatabolic Reductive Dehalogenase Substrate Complex Structures Underpin Rational Repurposing of Substrate Scope.
Journal, issue, pagesMicroorganisms, Vol. 8, Year 2020
Publish dateJul 30, 2020 (structure data deposition date)
AuthorsHalliwell, T. / Fisher, K. / Payne, K.A.P. / Rigby, S.E.J. / Leys, D.
External linksMicroorganisms / PubMed:32887524
MethodsX-ray diffraction
Resolution1.73 - 2.13 Å
Structure data

PDB-6zxu:
Catabolic reductive dehalogenase NpRdhA, N-terminally tagged.
Method: X-RAY DIFFRACTION / Resolution: 1.73 Å

PDB-6zxx:
Catabolic reductive dehalogenase NpRdhA, N-terminally tagged.
Method: X-RAY DIFFRACTION / Resolution: 1.99 Å

PDB-6zy0:
Catabolic reductive dehalogenase NpRdhA, N-terminally tagged, K488Q variant
Method: X-RAY DIFFRACTION / Resolution: 2.13 Å

PDB-6zy1:
Catabolic reductive dehalogenase NpRdhA, N-terminally tagged in complex with 3-bromo-4-hydroxybenzoic acid
Method: X-RAY DIFFRACTION / Resolution: 1.99 Å

Chemicals

ChemComp-SF4:
IRON/SULFUR CLUSTER

ChemComp-B12:
COBALAMIN

ChemComp-NA:
Unknown entry

ChemComp-CL:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-QSH:
3,5-bis(bromanyl)-4-oxidanyl-benzoic acid

ChemComp-QSB:
3 bromo 4 hydroxybenzoic acid

ChemComp-BR:
BROMIDE ION

Source
  • nitratireductor pacificus pht-3b (bacteria)
KeywordsOXIDOREDUCTASE / reductive dehalogenase / B12 / iron-sulfur

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