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Structure paper

TitleConformational Trapping of a beta-Glucosides-Binding Protein Unveils the Selective Two-Step Ligand-Binding Mechanism of ABC Importers.
Journal, issue, pagesJ. Mol. Biol., Vol. 432, Page 5711-5734, Year 2020
Publish dateMay 21, 2020 (structure data deposition date)
AuthorsChandravanshi, M. / Samanta, R. / Kanaujia, S.P.
External linksJ. Mol. Biol. / PubMed:32866452
MethodsX-ray diffraction
Resolution1.63 - 2.8 Å
Structure data

PDB-7c63:
Crystal structure of beta-glycosides-binding protein of ABC transporter in an open state (Form I)
Method: X-RAY DIFFRACTION / Resolution: 1.63 Å

PDB-7c64:
Crystal structure of beta-glycosides-binding protein of ABC transporter in an open state (Form II)
Method: X-RAY DIFFRACTION / Resolution: 1.63 Å

PDB-7c66:
Crystal structure of beta-glycosides-binding protein of ABC transporter in a closed state bound to cellobiose
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

PDB-7c67:
Crystal structure of beta-glycosides-binding protein of ABC transporter in a closed state bound to cellotriose
Method: X-RAY DIFFRACTION / Resolution: 2.0 Å

PDB-7c68:
Crystal structure of beta-glycosides-binding protein of ABC transporter in a closed state bound to cellotetraose
Method: X-RAY DIFFRACTION / Resolution: 2.05 Å

PDB-7c69:
Crystal structure of beta-glycosides-binding protein of ABC transporter in a closed state bound to sophorose
Method: X-RAY DIFFRACTION / Resolution: 2.35 Å

PDB-7c6f:
Crystal structure of beta-glycosides-binding protein (W177X) of ABC transporter in an open state
Method: X-RAY DIFFRACTION / Resolution: 1.7 Å

PDB-7c6g:
Crystal structure of beta-glycosides-binding protein (W177X) of ABC transporter in an open-liganded state bound to gentiobiose
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-7c6h:
Crystal structure of beta-glycosides-binding protein (W177X) of ABC transporter in an open-liganded state bound to laminaribiose
Method: X-RAY DIFFRACTION / Resolution: 1.85 Å

PDB-7c6i:
Crystal structure of beta-glycosides-binding protein (W177X) of ABC transporter in an open-liganded state bound to sophorose
Method: X-RAY DIFFRACTION / Resolution: 1.7 Å

PDB-7c6j:
Crystal structure of beta-glycosides-binding protein (W177X) of ABC transporter in a closed state bound to cellobiose
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-7c6k:
Crystal structure of beta-glycosides-binding protein (W177X) of ABC transporter in a closed state bound to cellotriose (Form I)
Method: X-RAY DIFFRACTION / Resolution: 1.77 Å

PDB-7c6l:
Crystal structure of beta-glycosides-binding protein (W177X) of ABC transporter in a closed state bound to cellotriose (Form II)
Method: X-RAY DIFFRACTION / Resolution: 2.4 Å

PDB-7c6m:
Crystal structure of beta-glycosides-binding protein (W177X) of ABC transporter in a closed state bound to cellotetraose (Form I)
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-7c6n:
Crystal structure of beta-glycosides-binding protein (W177X) of ABC transporter in a closed state bound to cellotetraose (Form II)
Method: X-RAY DIFFRACTION / Resolution: 2.05 Å

PDB-7c6r:
Crystal structure of beta-glycosides-binding protein (W177X) of ABC transporter in a closed state bound to cellopentaose
Method: X-RAY DIFFRACTION / Resolution: 1.96 Å

PDB-7c6t:
Crystal structure of beta-glycosides-binding protein (W177X) of ABC transporter in a closed state bound to laminaritriose (Form I)
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

PDB-7c6v:
Crystal structure of beta-glycosides-binding protein (W177X) of ABC transporter in a closed state bound to laminaritriose (Form II)
Method: X-RAY DIFFRACTION / Resolution: 2.8 Å

PDB-7c6w:
Crystal structure of beta-glycosides-binding protein (W177X) of ABC transporter in a closed state bound to laminaritetraose
Method: X-RAY DIFFRACTION / Resolution: 1.88 Å

PDB-7c6x:
Crystal structure of beta-glycosides-binding protein (W41A) of ABC transporter in an open state (Form I)
Method: X-RAY DIFFRACTION / Resolution: 2.65 Å

PDB-7c6y:
Crystal structure of beta-glycosides-binding protein (W41A) of ABC transporter in an open state (Form II)
Method: X-RAY DIFFRACTION / Resolution: 1.7 Å

PDB-7c6z:
Crystal structure of beta-glycosides-binding protein (W67A) of ABC transporter in an open state
Method: X-RAY DIFFRACTION / Resolution: 1.63 Å

PDB-7c70:
Crystal structure of beta-glycosides-binding protein (W67A) of ABC transporter in an open-liganded state bound to gentiobiose
Method: X-RAY DIFFRACTION / Resolution: 1.63 Å

PDB-7c71:
Crystal structure of beta-glycosides-binding protein (E117A) of ABC transporter in an open state
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

Chemicals

ChemComp-CL:
Unknown entry / Chloride

ChemComp-SO3:
SULFITE ION / Sulfite

ChemComp-SO4:
SULFATE ION / Sulfate

ChemComp-EDO:
1,2-ETHANEDIOL / Ethylene glycol

ChemComp-BGQ:
2-HYDROXY BUTANE-1,4-DIOL

ChemComp-GOL:
GLYCEROL / Glycerol

ChemComp-HOH:
WATER / Water

ChemComp-CO2:
CARBON DIOXIDE / Carbon dioxide

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER / Diethylene glycol

ChemComp-PGE:
TRIETHYLENE GLYCOL / Polyethylene glycol

ChemComp-PG4:
TETRAETHYLENE GLYCOL / precipitant*YM / Polyethylene glycol

ChemComp-MRY:
MESO-ERYTHRITOL / Erythritol

ChemComp-PO4:
PHOSPHATE ION / Phosphate

ChemComp-P33:
3,6,9,12,15,18-HEXAOXAICOSANE-1,20-DIOL / precipitant*YM / Polyethylene glycol

ChemComp-SO2:
SULFUR DIOXIDE / Sulfur dioxide

ChemComp-NA:
Unknown entry

ChemComp-CO3:
CARBONATE ION / Carbonate

ChemComp-ACT:
ACETATE ION / Acetate

ChemComp-PGO:
S-1,2-PROPANEDIOL / Propanediol

ChemComp-BU2:
1,3-BUTANEDIOL / 1,3-Butanediol

ChemComp-FJO:
propane-1,1,1,3-tetrol

ChemComp-POP:
PYROPHOSPHATE 2- / Pyrophosphate

ChemComp-MPD:
(4S)-2-METHYL-2,4-PENTANEDIOL / precipitant*YM / 2-Methyl-2,4-pentanediol

ChemComp-IMD:
IMIDAZOLE / Imidazole

Source
  • thermus thermophilus (strain hb8 / atcc 27634 / dsm 579) (bacteria)
  • thermus thermophilus hb8 (bacteria)
KeywordsSUGAR BINDING PROTEIN / Conformational dynamics / substrate-binding protein / Induced-fit mechanism / Two-step ligand binding / Venus Fly-trap mechanism

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