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-Structure paper
タイトル | A molecular pore spans the double membrane of the coronavirus replication organelle. |
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ジャーナル・号・ページ | Science, Vol. 369, Issue 6509, Page 1395-1398, Year 2020 |
掲載日 | 2020年9月11日 |
著者 | Georg Wolff / Ronald W A L Limpens / Jessika C Zevenhoven-Dobbe / Ulrike Laugks / Shawn Zheng / Anja W M de Jong / Roman I Koning / David A Agard / Kay Grünewald / Abraham J Koster / Eric J Snijder / Montserrat Bárcena / |
PubMed 要旨 | Coronavirus genome replication is associated with virus-induced cytosolic double-membrane vesicles, which may provide a tailored microenvironment for viral RNA synthesis in the infected cell. ...Coronavirus genome replication is associated with virus-induced cytosolic double-membrane vesicles, which may provide a tailored microenvironment for viral RNA synthesis in the infected cell. However, it is unclear how newly synthesized genomes and messenger RNAs can travel from these sealed replication compartments to the cytosol to ensure their translation and the assembly of progeny virions. In this study, we used cellular cryo-electron microscopy to visualize a molecular pore complex that spans both membranes of the double-membrane vesicle and would allow export of RNA to the cytosol. A hexameric assembly of a large viral transmembrane protein was found to form the core of the crown-shaped complex. This coronavirus-specific structure likely plays a key role in coronavirus replication and thus constitutes a potential drug target. |
リンク | Science / PubMed:32763915 / PubMed Central |
手法 | EM (サブトモグラム平均) |
解像度 | 30.5 - 40.5 Å |
構造データ | EMDB-11514: EMDB-11515: |
由来 |
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