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Structure paper

TitleTrapping conformational states of a flavin-dependent N -monooxygenase in crystallo reveals protein and flavin dynamics.
Journal, issue, pagesJ. Biol. Chem., Vol. 295, Page 13239-13249, Year 2020
Publish dateMay 15, 2020 (structure data deposition date)
AuthorsCampbell, A.C. / Stiers, K.M. / Martin Del Campo, J.S. / Mehra-Chaudhary, R. / Sobrado, P. / Tanner, J.J.
External linksJ. Biol. Chem. / PubMed:32723870
MethodsX-ray diffraction
Resolution1.95 - 2.335 Å
Structure data

PDB-6x0h:
Structure of oxidized SidA ornithine hydroxylase with the FAD in the "out" conformation
Method: X-RAY DIFFRACTION / Resolution: 2.087 Å

PDB-6x0i:
Structure of oxidized SidA ornithine hydroxylase with the FAD "in" and complexed with NADP
Method: X-RAY DIFFRACTION / Resolution: 1.95 Å

PDB-6x0j:
Structure of reduced SidA ornithine hydroxylase with the FAD "in" and complexed with NADP and L-ornithine
Method: X-RAY DIFFRACTION / Resolution: 2.335 Å

PDB-6x0k:
Structure of dithionite-reduced SidA ornithine hydroxylase with the FAD "in" and complexed with L-ornithine
Method: X-RAY DIFFRACTION / Resolution: 2.231 Å

Chemicals

ChemComp-FAD:
FLAVIN-ADENINE DINUCLEOTIDE / FAD*YM

ChemComp-ACT:
ACETATE ION

ChemComp-PGE:
TRIETHYLENE GLYCOL

ChemComp-CA:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-NAP:
NADP NICOTINAMIDE-ADENINE-DINUCLEOTIDE PHOSPHATE

ChemComp-ORN:
L-ornithine

ChemComp-FDA:
DIHYDROFLAVINE-ADENINE DINUCLEOTIDE

Source
  • aspergillus fumigatus (mold)
  • neosartorya fumigata (strain atcc mya-4609 / af293 / cbs 101355 / fgsc a1100) (mold)
KeywordsOXIDOREDUCTASE / FLAVIN-CONTAINING MONOOXYGENASE / FLAVOPROTEIN

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