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TitleCold sensitivity of the SARS-CoV-2 spike ectodomain.
Journal, issue, pagesbioRxiv, Year 2020
Publish dateOct 13, 2020
AuthorsRobert J Edwards / Katayoun Mansouri / Victoria Stalls / Kartik Manne / Brian Watts / Rob Parks / Katarzyna Janowska / Sophie M C Gobeil / Megan Kopp / Dapeng Li / Xiaozhi Lu / Zekun Mu / Margaret Deyton / Thomas H Oguin / Jordan Sprenz / Wilton Williams / Kevin Saunders / David Montefiori / Gregory D Sempowski / Rory Henderson / Munir Alam / Barton F Haynes / Priyamvada Acharya
PubMed AbstractThe SARS-CoV-2 spike (S) protein, a primary target for COVID-19 vaccine development, presents its Receptor Binding Domain in two conformations: receptor-accessible "up" or receptor-inaccessible ...The SARS-CoV-2 spike (S) protein, a primary target for COVID-19 vaccine development, presents its Receptor Binding Domain in two conformations: receptor-accessible "up" or receptor-inaccessible "down" conformations. Here, we report that the commonly used stabilized S ectodomain construct "2P" is sensitive to cold temperature, and that this cold sensitivity is resolved in a "down" state stabilized spike. Our results will impact structural, functional and vaccine studies that use the SARS-CoV-2 S ectodomain.
External linksbioRxiv / PubMed:32699852 / PubMed Central
MethodsEM (single particle)
Resolution10.7 - 16.8 Å
Structure data

EMDB-22934:
Negative stain electron microscopy reconstruction of rS2d-hexapro SARS-CoV-2 spike ectodomain
Method: EM (single particle) / Resolution: 10.7 Å

EMDB-22967:
Negative stain electron microscopy reconstruction of 2P SARS-CoV-2 spike ectodomain in the 3-RBD-down state after cold-denaturation at 4 degrees C for one week followed by re-naturation at 37 degrees C for 3 hours.
Method: EM (single particle) / Resolution: 16.8 Å

EMDB-22968:
Negative stain electron microscopy reconstruction of 2P SARS-CoV-2 spike ectodomain in the 1-RBD-up state after cold-denaturation at 4 degrees C for one week followed by re-naturation at 37 degrees C for 3 hours.
Method: EM (single particle) / Resolution: 16.1 Å

Source
  • Severe acute respiratory syndrome coronavirus 2

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