Movie
Controller
Structure viewers
About Yorodumi Papers
+Search query
-Structure paper
| Title | Near-atomic structures of the BBSome reveal the basis for BBSome activation and binding to GPCR cargoes. |
|---|---|
| Journal, issue, pages | Elife, Vol. 9, Year 2020 |
| Publish date | Jun 8, 2020 |
 Authors | Shuang Yang / Kriti Bahl / Hui-Ting Chou / Jonathan Woodsmith / Ulrich Stelzl / Thomas Walz / Maxence V Nachury /   |
| PubMed Abstract | Dynamic trafficking of G protein-coupled receptors (GPCRs) out of cilia is mediated by the BBSome. In concert with its membrane recruitment factor, the small GTPase ARL6/BBS3, the BBSome ferries ...Dynamic trafficking of G protein-coupled receptors (GPCRs) out of cilia is mediated by the BBSome. In concert with its membrane recruitment factor, the small GTPase ARL6/BBS3, the BBSome ferries GPCRs across the transition zone, a diffusion barrier at the base of cilia. Here, we present the near-atomic structures of the BBSome by itself and in complex with ARL6, and we describe the changes in BBSome conformation induced by ARL6 binding. Modeling the interactions of the BBSome with membranes and the GPCR Smoothened (SMO) reveals that SMO, and likely also other GPCR cargoes, must release their amphipathic helix 8 from the membrane to be recognized by the BBSome. |
 External links | Elife / PubMed:32510327 / PubMed Central |
| Methods | EM (single particle) |
| Resolution | 3.44 - 4.0 Å |
| Structure data | EMDB-21251, PDB-6vnw: EMDB-21259, PDB-6voa: |
| Source |
|
 Keywords |  PROTEIN TRANSPORT / Cilia / Bardet-Biedl Syndrome |
