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Structure paper

TitleStructure of an ancestral mammalian family 1B1 cytochrome P450 with increased thermostability.
Journal, issue, pagesJ. Biol. Chem., Vol. 295, Page 5640-5653, Year 2020
Publish dateMay 15, 2019 (structure data deposition date)
AuthorsBart, A.G. / Harris, K.L. / Gillam, E.M.J. / Scott, E.E.
External linksJ. Biol. Chem. / PubMed:32156703
MethodsX-ray diffraction
Resolution2.95 - 3.101 Å
Structure data

PDB-6oyu:
Structure of an ancestral-reconstructed cytochrome P450 1B1 with alpha-naphthoflavone
Method: X-RAY DIFFRACTION / Resolution: 2.95 Å

PDB-6oyv:
Structure of an ancestral-reconstructed cytochrome P450 1B1 with estradiol
Method: X-RAY DIFFRACTION / Resolution: 3.101 Å

Chemicals

ChemComp-BHF:
2-PHENYL-4H-BENZO[H]CHROMEN-4-ONE / inhibitor*YM

ChemComp-HEM:
PROTOPORPHYRIN IX CONTAINING FE

ChemComp-GOL:
GLYCEROL

ChemComp-HOH:
WATER

ChemComp-EST:
ESTRADIOL / hormone*YM

Source
  • synthetic construct (others)
KeywordsOXIDOREDUCTASE / CYP1B1 / ancestral reconstruction / mammalian

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