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Title | Polymorphic Protective Dps-DNA Co-Crystals by Cryo Electron Tomography and Small Angle X-Ray Scattering. |
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Journal, issue, pages | Biomolecules, Vol. 10, Issue 1, Year 2019 |
Publish date | Dec 26, 2019 |
![]() | Roman Kamyshinsky / Yury Chesnokov / Liubov Dadinova / Andrey Mozhaev / Ivan Orlov / Maxim Petoukhov / Anton Orekhov / Eleonora Shtykova / Alexander Vasiliev / ![]() |
PubMed Abstract | Rapid increase of intracellular synthesis of specific histone-like Dps protein that binds DNA to protect the genome against deleterious factors leads to in cellulo crystallization-one of the most ...Rapid increase of intracellular synthesis of specific histone-like Dps protein that binds DNA to protect the genome against deleterious factors leads to in cellulo crystallization-one of the most curious processes in the area of life science at the moment. However, the actual structure of the Dps-DNA co-crystals remained uncertain in the details for more than two decades. Cryo-electron tomography and small-angle X-ray scattering revealed polymorphous modifications of the co-crystals depending on the buffer parameters. Two different types of the Dps-DNA co-crystals are formed in vitro: triclinic and cubic. Three-dimensional reconstruction revealed DNA and Dps molecules in cubic co-crystals, and the unit cell parameters of cubic lattice were determined consistently by both methods. |
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Methods | EM (subtomogram averaging) |
Resolution | 13.7 Å |
Structure data | ![]() EMDB-10286: |
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