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-Structure paper
タイトル | Electron cryotomography of intact motile cilia defines the basal body to axoneme transition. |
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ジャーナル・号・ページ | J Cell Biol, Vol. 219, Issue 1, Year 2020 |
掲載日 | 2020年1月6日 |
著者 | Garrett A Greenan / Ronald D Vale / David A Agard / |
PubMed 要旨 | Cells use motile cilia to generate force in the extracellular space. The structure of a cilium can be classified into three subdomains: the intracellular basal body (BB) that templates cilium ...Cells use motile cilia to generate force in the extracellular space. The structure of a cilium can be classified into three subdomains: the intracellular basal body (BB) that templates cilium formation, the extracellular axoneme that generates force, and the transition zone (TZ) that bridges them. While the BB is composed of triplet microtubules (TMTs), the axoneme is composed of doublet microtubules (DMTs), meaning the cilium must convert between different microtubule geometries. Here, we performed electron cryotomography to define this conversion, and our reconstructions reveal identifying structural features of the BB, TZ, and axoneme. Each region is distinct in terms of microtubule number and geometry, microtubule inner proteins, and microtubule linkers. TMT to DMT conversion occurs within the BB, and microtubule geometry changes to axonemal by the end of the TZ, followed by the addition of axoneme-specific components essential for cilium motility. Our results provide the highest-resolution images of the motile cilium to date and reveal how BBs template axonemes. |
リンク | J Cell Biol / PubMed:31874113 / PubMed Central |
手法 | EM (サブトモグラム平均) |
解像度 | 30.7 - 35.6 Å |
構造データ | EMDB-20674: EMDB-20675: EMDB-20676: EMDB-20679: EMDB-20680: EMDB-20681: EMDB-20683: EMDB-20684: EMDB-20685: |
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