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TitleA 3.3 Å-Resolution Structure of Hyperthermophilic Respiratory Complex III Reveals the Mechanism of Its Thermal Stability.
Journal, issue, pagesAngew Chem Int Ed Engl, Vol. 59, Issue 1, Page 343-351, Year 2020
Publish dateJan 2, 2020
AuthorsGuoliang Zhu / Hui Zeng / Shuangbo Zhang / Jana Juli / Xiaoyun Pang / Jan Hoffmann / Yan Zhang / Nina Morgner / Yun Zhu / Guohong Peng / Hartmut Michel / Fei Sun /
PubMed AbstractRespiratory chain complexes convert energy by coupling electron flow to transmembrane proton translocation. Owing to a lack of atomic structures of cytochrome bc complex (Complex III) from ...Respiratory chain complexes convert energy by coupling electron flow to transmembrane proton translocation. Owing to a lack of atomic structures of cytochrome bc complex (Complex III) from thermophilic bacteria, little is known about the adaptations of this macromolecular machine to hyperthermophilic environments. In this study, we purified the cytochrome bc complex of Aquifex aeolicus, one of the most extreme thermophilic bacteria known, and determined its structure with and without an inhibitor at 3.3 Å resolution. Several residues unique for thermophilic bacteria were detected that provide additional stabilization for the structure. An extra transmembrane helix at the N-terminus of cyt. c was found to greatly enhance the interaction between cyt. b and cyt. c , and to bind a phospholipid molecule to stabilize the complex in the membrane. These results provide the structural basis for the hyperstability of the cytochrome bc complex in an extreme thermal environment.
External linksAngew Chem Int Ed Engl / PubMed:31778296 / PubMed Central
MethodsEM (single particle)
Resolution3.2 - 3.3 Å
Structure data

0716

6kls

EMDB-0716, PDB-6kls:
Hyperthermophilic respiratory Complex III
Method: EM (single particle) / Resolution: 3.3 Å

0719

6klv

EMDB-0719, PDB-6klv:
Hyperthermophilic respiratory Complex III
Method: EM (single particle) / Resolution: 3.2 Å

Chemicals

ChemComp-FES:
FE2/S2 (INORGANIC) CLUSTER / Iron–sulfur cluster

ChemComp-HEM:
PROTOPORPHYRIN IX CONTAINING FE / Heme B

ChemComp-DLX:
2-[(2~{E},6~{E},10~{Z},14~{Z},18~{Z},23~{R})-3,7,11,15,19,23,27-heptamethyloctacosa-2,6,10,14,18-pentaenyl]naphthalene-1,4-dione

ChemComp-PGV:
(1R)-2-{[{[(2S)-2,3-DIHYDROXYPROPYL]OXY}(HYDROXY)PHOSPHORYL]OXY}-1-[(PALMITOYLOXY)METHYL]ETHYL (11E)-OCTADEC-11-ENOATE / phospholipid*YM / Phosphatidylglycerol

ChemComp-HEC:
HEME C / Heme C

ChemComp-AMY:
ANTIMYCIN / Antimycin

Source
  • aquifex aeolicus (bacteria)
  • aquifex aeolicus (strain vf5) (bacteria)
KeywordsOXIDOREDUCTASE / Respiratory chain / Complex III / Hyperthermophilic mechanism / antimycin A

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