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Structure paper

TitleMglA functions as a three-state GTPase to control movement reversals of Myxococcus xanthus.
Journal, issue, pagesNat Commun, Vol. 10, Page 5300-5300, Year 2019
Publish dateJul 11, 2018 (structure data deposition date)
AuthorsGalicia, C. / Lhospice, S. / Varela, P.F. / Trapani, S. / Zhang, W. / Navaza, J. / Herrou, J. / Mignot, T. / Cherfils, J.
External linksNat Commun / PubMed:31757955
MethodsX-ray diffraction
Resolution1.275 - 3.3 Å
Structure data

PDB-6h17:
Myxococcus xanthus MglA bound to GTPgammaS
Method: X-RAY DIFFRACTION / Resolution: 1.275 Å

PDB-6h35:
Myxococcus xanthus MglA bound to GDP and Pi with mixed inactive and active switch region conformations
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

PDB-6h5b:
Myxococcus xanthus MglA in complex with its GAP MglB and GTPgammaS
Method: X-RAY DIFFRACTION / Resolution: 2.8 Å

PDB-6hjh:
Myxococcus xanthus MglA bound to GDP
Method: X-RAY DIFFRACTION / Resolution: 3.3 Å

PDB-6hjm:
Myxococcus xanthus MglB
Method: X-RAY DIFFRACTION / Resolution: 2.39 Å

PDB-6hjo:
Myxococcus xanthus MglA bound to GDP
Method: X-RAY DIFFRACTION / Resolution: 1.98 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-GSP:
5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE

ChemComp-SO4:
SULFATE ION

ChemComp-HOH:
WATER

ChemComp-GDP:
GUANOSINE-5'-DIPHOSPHATE / GDP, energy-carrying molecule*YM

ChemComp-PO4:
PHOSPHATE ION

ChemComp-MPD:
(4S)-2-METHYL-2,4-PENTANEDIOL / precipitant*YM

ChemComp-EPE:
4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID / pH buffer*YM

ChemComp-1PE:
PENTAETHYLENE GLYCOL / precipitant*YM

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

Source
  • myxococcus xanthus dk 1622 (bacteria)
  • myxococcus xanthus (bacteria)
KeywordsCYTOSOLIC PROTEIN / GTPase / motility / small GTPase in a mixed state / GTPase and GAP complex / Small GTPase / guanyl-nucleotide exchange factor (GEF)

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