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Structure paper

TitleEngineering a surrogate human heteromeric alpha / beta glycine receptor orthosteric site exploiting the structural homology and stability of acetylcholine-binding protein.
Journal, issue, pagesIucrj, Vol. 6, Page 1014-1023, Year 2019
Publish dateJun 12, 2017 (structure data deposition date)
AuthorsDawson, A. / Trumper, P. / de Souza, J.O. / Parker, H. / Jones, M.J. / Hales, T.G. / Hunter, W.N.
External linksIucrj / PubMed:31709057
MethodsX-ray diffraction
Resolution2 - 3.2 Å
Structure data

PDB-5o87:
Crystal structure of wild type Aplysia californica AChBP in complex with nicotine
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

PDB-5o8t:
Crystal structure of wild type Aplysia californica AChBP in complex with strychnine
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

PDB-5oa0:
Crystal structure of mutant AChBP in complex with strychnine (T53F, Q74R, Y110A, I135S, W164F)
Method: X-RAY DIFFRACTION / Resolution: 2.6 Å

PDB-5oad:
Crystal structure of mutant AChBP in complex with HEPES (T53F, Q74R, Y110A, I135S, G162E)
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-5oaj:
Crystal structure of mutant AChBP in complex with tropisetron (T53F, Q74R, Y110A, I135S, G162E)
Method: X-RAY DIFFRACTION / Resolution: 2.47 Å

PDB-5oal:
Crystal structure of mutant AChBP in complex with strychnine (T53F, Q74R, Y110A, I135S, G162E)
Method: X-RAY DIFFRACTION / Resolution: 3.2 Å

PDB-5oan:
Crystal structure of mutant AChBP in complex with glycine (T53F, Q74R, Y110A, I135S, G162E, S206CCP_KGTG)
Method: X-RAY DIFFRACTION / Resolution: 2.6 Å

PDB-5obg:
Crystal structure of glycine binding protein in complex with strychnine
Method: X-RAY DIFFRACTION / Resolution: 2.0 Å

Chemicals

ChemComp-NCT:
(S)-3-(1-METHYLPYRROLIDIN-2-YL)PYRIDINE / alkaloid*YM / Nicotine

ChemComp-NAG:
2-acetamido-2-deoxy-beta-D-glucopyranose / N-Acetylglucosamine

ChemComp-EDO:
1,2-ETHANEDIOL / Ethylene glycol

ChemComp-PO4:
PHOSPHATE ION / Phosphate

ChemComp-IPA:
ISOPROPYL ALCOHOL / alkaloid*YM / Isopropyl alcohol

ChemComp-HOH:
WATER / Water

ChemComp-SY9:
STRYCHNINE / alkaloid*YM / Strychnine

ChemComp-EPE:
4-(2-HYDROXYETHYL)-1-PIPERAZINE ETHANESULFONIC ACID / pH buffer*YM / HEPES

ChemComp-FLC:
CITRATE ANION / Citric acid

ChemComp-TKT:
(3-ENDO)-8-METHYL-8-AZABICYCLO[3.2.1]OCT-3-YL 1H-INDOLE-3-CARBOXYLATE / chemotherapy, antagonist*YM / Tropisetron

ChemComp-ARG:
ARGININE / Arginine

ChemComp-CL:
Unknown entry / Chloride

ChemComp-ACT:
ACETATE ION / Acetate

ChemComp-GLY:
GLYCINE / Glycine

ChemComp-NA:
Unknown entry

Source
  • aplysia californica (California sea hare)
KeywordsSIGNALING PROTEIN / receptor / acetylcholine binding

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