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Structure paper

TitleA cryptic hydrophobic pocket in the polo-box domain of the polo-like kinase PLK1 regulates substrate recognition and mitotic chromosome segregation.
Journal, issue, pagesSci Rep, Vol. 9, Page 15930-15930, Year 2019
Publish dateMar 16, 2017 (structure data deposition date)
AuthorsSharma, P. / Mahen, R. / Rossmann, M. / Stokes, J.E. / Hardwick, B. / Huggins, D.J. / Emery, A. / Kunciw, D.L. / Hyvonen, M. / Spring, D.R. ...Sharma, P. / Mahen, R. / Rossmann, M. / Stokes, J.E. / Hardwick, B. / Huggins, D.J. / Emery, A. / Kunciw, D.L. / Hyvonen, M. / Spring, D.R. / McKenzie, G.J. / Venkitaraman, A.R.
External linksSci Rep / PubMed:31685831
MethodsX-ray diffraction
Resolution1.785 - 2.02 Å
Structure data

PDB-5nfu:
The structure of the polo-box domain (PBD) of polo-like kinase 1 (Plk1) in complex with LHSpTA peptide
Method: X-RAY DIFFRACTION / Resolution: 1.809 Å

PDB-5nje:
The structure of the polo-box domain (PBD) of polo-like kinase 1 (Plk1) in complex with Alpha-Bromo-3-Iodotoluene.
Method: X-RAY DIFFRACTION / Resolution: 1.978 Å

PDB-5nmm:
The structure of the polo-box domain (PBD) of Plk1 in complex with Alpha-Bromo-3-Iodotoluene.
Method: X-RAY DIFFRACTION / Resolution: 2.02 Å

PDB-5nn1:
The structure of the polo-box domain (PBD) of polo-like kinase 1 (Plk1)
Method: X-RAY DIFFRACTION / Resolution: 1.785 Å

PDB-5nn2:
The structure of the polo-box domain (PBD) of Plk1 in complex with Z228588490
Method: X-RAY DIFFRACTION / Resolution: 1.81 Å

Chemicals

ChemComp-HOH:
WATER

ChemComp-8Z5:
Alpha-Bromo-3-Iodotoluene

ChemComp-Z24:
(3~{R},5~{R})-1-[2,4-bis(fluoranyl)phenyl]-5-oxidanyl-pyrrolidine-3-carboxylic acid

Source
  • homo sapiens (human)
KeywordsTRANSFERASE / PLK1 / PBD1 / mitosis / small molecule

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