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TitleStructure of a P element transposase-DNA complex reveals unusual DNA structures and GTP-DNA contacts.
Journal, issue, pagesNat Struct Mol Biol, Vol. 26, Issue 11, Page 1013-1022, Year 2019
Publish dateOct 28, 2019
AuthorsGeorge E Ghanim / Elizabeth H Kellogg / Eva Nogales / Donald C Rio /
PubMed AbstractP element transposase catalyzes the mobility of P element DNA transposons within the Drosophila genome. P element transposase exhibits several unique properties, including the requirement for a ...P element transposase catalyzes the mobility of P element DNA transposons within the Drosophila genome. P element transposase exhibits several unique properties, including the requirement for a guanosine triphosphate cofactor and the generation of long staggered DNA breaks during transposition. To gain insights into these features, we determined the atomic structure of the Drosophila P element transposase strand transfer complex using cryo-EM. The structure of this post-transposition nucleoprotein complex reveals that the terminal single-stranded transposon DNA adopts unusual A-form and distorted B-form helical geometries that are stabilized by extensive protein-DNA interactions. Additionally, we infer that the bound guanosine triphosphate cofactor interacts with the terminal base of the transposon DNA, apparently to position the P element DNA for catalysis. Our structure provides the first view of the P element transposase superfamily, offers new insights into P element transposition and implies a transposition pathway fundamentally distinct from other cut-and-paste DNA transposases.
External linksNat Struct Mol Biol / PubMed:31659330 / PubMed Central
MethodsEM (single particle)
Resolution3.6 - 4.0 Å
Structure data

20254

6p5a

EMDB-20254, PDB-6p5a:
Drosophila P element transposase strand transfer complex
Method: EM (single particle) / Resolution: 3.6 Å

20321

6pe2

EMDB-20321: asymmetric
PDB-6pe2: Drosophila P element transposase strand transfer complex
Method: EM (single particle) / Resolution: 4.0 Å

Chemicals

ChemComp-GTP:
GUANOSINE-5'-TRIPHOSPHATE / GTP, energy-carrying molecule*YM / Guanosine triphosphate

ChemComp-MG:
Unknown entry

Source
  • drosophila melanogaster (fruit fly)
KeywordsTRANSFERASE/DNA / transposase / Strand Transfer Complex / TRANSFERASE-DNA complex / TRANSFERASE-DNA

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