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-Structure paper
タイトル | A packing for A-form DNA in an icosahedral virus. |
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ジャーナル・号・ページ | Proc Natl Acad Sci U S A, Vol. 116, Issue 45, Page 22591-22597, Year 2019 |
掲載日 | 2019年11月5日 |
著者 | Fengbin Wang / Ying Liu / Zhangli Su / Tomasz Osinski / Guilherme A P de Oliveira / James F Conway / Stefan Schouten / Mart Krupovic / David Prangishvili / Edward H Egelman / |
PubMed 要旨 | Studies on viruses infecting archaea living in the most extreme environments continue to show a remarkable diversity of structures, suggesting that the sampling continues to be very sparse. We have ...Studies on viruses infecting archaea living in the most extreme environments continue to show a remarkable diversity of structures, suggesting that the sampling continues to be very sparse. We have used electron cryo-microscopy to study at 3.7-Å resolution the structure of the polyhedral virus 1 (SPV1), which was originally isolated from a hot, acidic spring in Beppu, Japan. The 2 capsid proteins with variant single jelly-roll folds form pentamers and hexamers which assemble into a = 43 icosahedral shell. In contrast to tailed icosahedral double-stranded DNA (dsDNA) viruses infecting bacteria and archaea, and herpesviruses infecting animals and humans, where naked DNA is packed under very high pressure due to the repulsion between adjacent layers of DNA, the circular dsDNA in SPV1 is fully covered with a viral protein forming a nucleoprotein filament with attractive interactions between layers. Most strikingly, we have been able to show that the DNA is in an A-form, as it is in the filamentous viruses infecting hyperthermophilic acidophiles. Previous studies have suggested that DNA is in the B-form in bacteriophages, and our study is a direct visualization of the structure of DNA in an icosahedral virus. |
リンク | Proc Natl Acad Sci U S A / PubMed:31636205 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 3.7 Å |
構造データ | EMDB-20083, PDB-6oj0: |
由来 |
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キーワード | VIRUS / archaeal pilus / STRUCTURAL PROTEIN / icosahedral symmetry |