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Structure paper

TitleA lipid site shapes the agonist response of a pentameric ligand-gated ion channel.
Journal, issue, pagesNat. Chem. Biol., Vol. 15, Page 1156-1164, Year 2019
Publish dateSep 4, 2018 (structure data deposition date)
AuthorsHenault, C.M. / Govaerts, C. / Spurny, R. / Brams, M. / Estrada-Mondragon, A. / Lynch, J. / Bertrand, D. / Pardon, E. / Evans, G.L. / Woods, K. ...Henault, C.M. / Govaerts, C. / Spurny, R. / Brams, M. / Estrada-Mondragon, A. / Lynch, J. / Bertrand, D. / Pardon, E. / Evans, G.L. / Woods, K. / Elberson, B.W. / Cuello, L.G. / Brannigan, G. / Nury, H. / Steyaert, J. / Baenziger, J.E. / Ulens, C.
External linksNat. Chem. Biol. / PubMed:31591563
MethodsX-ray diffraction
Resolution2.5 - 3.45 Å
Structure data

PDB-6hjx:
X-ray structure of a pentameric ligand gated ion channel from Erwinia chrysanthemi (ELIC) 7'C pore mutant (L238C) in complex with nanobody 72
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

PDB-6hjy:
X-ray structure of a pentameric ligand gated ion channel from Erwinia chrysanthemi (ELIC) Delta8 truncation mutant in complex with nanobody 72
Method: X-RAY DIFFRACTION / Resolution: 2.78 Å

PDB-6hk0:
X-ray structure of a pentameric ligand gated ion channel from Erwinia chrysanthemi (ELIC) F16'S pore mutant (F247S) with alternate M4 conformation.
Method: X-RAY DIFFRACTION / Resolution: 3.45 Å

Chemicals

ChemComp-P6G:
HEXAETHYLENE GLYCOL / precipitant*YM

ChemComp-PTY:
PHOSPHATIDYLETHANOLAMINE / phospholipid*YM

ChemComp-LMT:
DODECYL-BETA-D-MALTOSIDE / detergent*YM

ChemComp-MES:
2-(N-MORPHOLINO)-ETHANESULFONIC ACID / pH buffer*YM

ChemComp-NA:
Unknown entry

ChemComp-HOH:
WATER

Source
  • dickeya chrysanthemi (bacteria)
  • lama glama (llama)
KeywordsMEMBRANE PROTEIN / ion channel / pentameric ligand-gated ion channel / cya-loop receptor

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