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Structure paper

TitleMicroED with the Falcon III direct electron detector.
Journal, issue, pagesIUCrJ, Vol. 6, Issue Pt 5, Page 921-926, Year 2019
Publish dateSep 1, 2019
AuthorsJohan Hattne / Michael W Martynowycz / Pawel A Penczek / Tamir Gonen /
PubMed AbstractMicrocrystal electron diffraction (MicroED) combines crystallography and electron cryo-microscopy (cryo-EM) into a method that is applicable to high-resolution structure determination. In MicroED, ...Microcrystal electron diffraction (MicroED) combines crystallography and electron cryo-microscopy (cryo-EM) into a method that is applicable to high-resolution structure determination. In MicroED, nanosized crystals, which are often intractable using other techniques, are probed by high-energy electrons in a transmission electron microscope. Diffraction data are recorded by a camera in movie mode: the nanocrystal is continuously rotated in the beam, thus creating a sequence of frames that constitute a movie with respect to the rotation angle. Until now, diffraction-optimized cameras have mostly been used for MicroED. Here, the use of a direct electron detector that was designed for imaging is reported. It is demonstrated that data can be collected more rapidly using the Falcon III for MicroED and with markedly lower exposure than has previously been reported. The Falcon III was operated at 40 frames per second and complete data sets reaching atomic resolution were recorded in minutes. The resulting density maps to 2.1 Å resolution of the serine protease proteinase K showed no visible signs of radiation damage. It is thus demonstrated that dedicated diffraction-optimized detectors are not required for MicroED, as shown by the fact that the very same cameras that are used for imaging applications in electron microscopy, such as single-particle cryo-EM, can also be used effectively for diffraction measurements.
External linksIUCrJ / PubMed:31576224 / PubMed Central
MethodsEM (electron crystallography)
Resolution2.1 - 2.7 Å
Structure data

20475

6pu4

EMDB-20475, PDB-6pu4:
MicroED structure of proteinase K recorded on Falcon III
Method: EM (electron crystallography) / Resolution: 2.1 Å

20476

6pu5

EMDB-20476, PDB-6pu5:
MicroED structure of proteinase K recorded on CetaD
Method: EM (electron crystallography) / Resolution: 2.7 Å

Chemicals

ChemComp-CA:
Unknown entry

ChemComp-HOH:
WATER / Water

Source
  • parengyodontium album (fungus)
KeywordsHYDROLASE

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