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Structure paper

TitleA polar filter in DNA polymerases prevents ribonucleotide incorporation.
Journal, issue, pagesNucleic Acids Res., Vol. 47, Page 10693-10705, Year 2019
Publish dateApr 15, 2019 (structure data deposition date)
AuthorsJohnson, M.K. / Kottur, J. / Nair, D.T.
External linksNucleic Acids Res. / PubMed:31544946
MethodsX-ray diffraction
Resolution1.78 - 2.74 Å
Structure data

PDB-6jul:
MsDpo4-DNA complex 1
Method: X-RAY DIFFRACTION / Resolution: 2.3 Å

PDB-6jum:
MsDpo4-DNA complex 2
Method: X-RAY DIFFRACTION / Resolution: 1.78 Å

PDB-6jun:
MsDpo4-DNA complex 3
Method: X-RAY DIFFRACTION / Resolution: 2.51 Å

PDB-6juo:
MsDpo4-DNA complex 4
Method: X-RAY DIFFRACTION / Resolution: 2.16 Å

PDB-6jup:
Mutant PolIV-DNA incoming nucleotide complex
Method: X-RAY DIFFRACTION / Resolution: 2.44 Å

PDB-6juq:
mutant PolIV-DNA incoming nucleotide complex 2
Method: X-RAY DIFFRACTION / Resolution: 2.74 Å

PDB-6jur:
MsDpo4-DNA complex 5
Method: X-RAY DIFFRACTION / Resolution: 2.06 Å

PDB-6jus:
MsDpo4-DNA complex 6
Method: X-RAY DIFFRACTION / Resolution: 2.5 Å

Chemicals

ChemComp-0KX:
2'-deoxy-5'-O-[(R)-hydroxy{[(R)-hydroxy(phosphonooxy)phosphoryl]amino}phosphoryl]cytidine

ChemComp-MG:
Unknown entry

ChemComp-HOH:
WATER

ChemComp-2TM:
5'-O-[(S)-hydroxy{[(S)-hydroxy(phosphonooxy)phosphoryl]methyl}phosphoryl]cytidine

ChemComp-MN:
Unknown entry

Source
  • mycobacterium smegmatis str. mc2 155 (bacteria)
  • escherichia coli (E. coli)
  • mycobacterium smegmatis (strain atcc 700084 / mc(2)155) (bacteria)
  • mycobacterium smegmatis (bacteria)
KeywordsREPLICATION/DNA / DNA polymerase / REPLICATION / REPLICATION-DNA complex

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