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TitleCryo-EM Structures of Azospirillum brasilense Glutamate Synthase in Its Oligomeric Assemblies.
Journal, issue, pagesJ Mol Biol, Vol. 431, Issue 22, Page 4523-4526, Year 2019
Publish dateNov 8, 2019
AuthorsPaolo Swuec / Antonio Chaves-Sanjuan / Carlo Camilloni / Maria Antonietta Vanoni / Martino Bolognesi /
PubMed AbstractBacterial NADPH-dependent glutamate synthase (GltS) is a complex iron-sulfur flavoprotein that catalyzes the reductive synthesis of two L-Glu molecules from L-Gln and 2-oxo-glutarate. GltS functional ...Bacterial NADPH-dependent glutamate synthase (GltS) is a complex iron-sulfur flavoprotein that catalyzes the reductive synthesis of two L-Glu molecules from L-Gln and 2-oxo-glutarate. GltS functional unit hosts an α-subunit (αGltS) and a β-subunit (βGltS) that assemble in different αβ oligomers in solution. Here, we present the cryo-electron microscopy structures of Azospirillum brasilense GltS in four different oligomeric states (αβ, αβ, αβ and αβ, in the 3.5- to 4.1-Å resolution range). Our study provides a comprehensive GltS model that details the inter-protomeric assemblies and allows unequivocal location of the FAD cofactor and of two electron transfer [4Fe-4S] clusters within βGltS.
External linksJ Mol Biol / PubMed:31473159
MethodsEM (single particle)
Resolution3.5 - 4.1 Å
Structure data

10104

6s6s

EMDB-10104, PDB-6s6s:
Structure of Azospirillum brasilense Glutamate Synthase in a4b4 oligomeric state.
Method: EM (single particle) / Resolution: 3.9 Å

10105

6s6t

EMDB-10105, PDB-6s6t:
Structure of Azospirillum brasilense Glutamate Synthase in a4b3 oligomeric state
Method: EM (single particle) / Resolution: 4.1 Å

10106

6s6u

EMDB-10106, PDB-6s6u:
Structure of Azospirillum brasilense Glutamate Synthase in a6b4 oligomeric state.
Method: EM (single particle) / Resolution: 3.5 Å

10108

6s6x

EMDB-10108, PDB-6s6x:
Structure of Azospirillum brasilense Glutamate Synthase in a6b6 oligomeric state.
Method: EM (single particle) / Resolution: 3.5 Å

Chemicals

ChemComp-FMN:
FLAVIN MONONUCLEOTIDE / Flavin mononucleotide

ChemComp-F3S:
FE3-S4 CLUSTER / Iron–sulfur cluster

ChemComp-SF4:
IRON/SULFUR CLUSTER / Iron–sulfur cluster

ChemComp-FAD:
FLAVIN-ADENINE DINUCLEOTIDE / FAD*YM / Flavin adenine dinucleotide

Source
  • azospirillum brasilense (bacteria)
KeywordsFLAVOPROTEIN / glutamate synthesys / complex / oligomeric assemblies

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