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TitleStructural insight into multistage inhibition of CRISPR-Cas12a by AcrVA4.
Journal, issue, pagesProc Natl Acad Sci U S A, Vol. 116, Issue 38, Page 18928-18936, Year 2019
Publish dateSep 17, 2019
AuthorsRuchao Peng / Zhiteng Li / Ying Xu / Shaoshuai He / Qi Peng / Lian-Ao Wu / Ying Wu / Jianxun Qi / Peiyi Wang / Yi Shi / George F Gao /
PubMed AbstractProkaryotes possess CRISPR-Cas systems to exclude parasitic predators, such as phages and mobile genetic elements (MGEs). These predators, in turn, encode anti-CRISPR (Acr) proteins to evade the ...Prokaryotes possess CRISPR-Cas systems to exclude parasitic predators, such as phages and mobile genetic elements (MGEs). These predators, in turn, encode anti-CRISPR (Acr) proteins to evade the CRISPR-Cas immunity. Recently, AcrVA4, an Acr protein inhibiting the CRISPR-Cas12a system, was shown to diminish Cas12a (LbCas12a)-mediated genome editing in human cells, but the underlying mechanisms remain elusive. Here we report the cryo-EM structures of AcrVA4 bound to CRISPR RNA (crRNA)-loaded LbCas12a and found AcrVA4 could inhibit LbCas12a at several stages of the CRISPR-Cas working pathway, different from other characterized type I/II Acr inhibitors which target only 1 stage. First, it locks the conformation of the LbCas12a-crRNA complex to prevent target DNA-crRNA hybridization. Second, it interacts with the LbCas12a-crRNA-dsDNA complex to release the bound DNA before cleavage. Third, AcrVA4 binds the postcleavage LbCas12a complex to possibly block enzyme recycling. These findings highlight the multifunctionality of AcrVA4 and provide clues for developing regulatory genome-editing tools.
External linksProc Natl Acad Sci U S A / PubMed:31467167 / PubMed Central
MethodsEM (single particle)
Resolution3.25 - 4.1 Å
Structure data

EMDB-0704, PDB-6kl9:
Structure of LbCas12a-crRNA complex bound to AcrVA4 (form A complex)
Method: EM (single particle) / Resolution: 3.25 Å

EMDB-0705, PDB-6klb:
Structure of LbCas12a-crRNA complex bound to AcrVA4 (form B complex)
Method: EM (single particle) / Resolution: 4.1 Å

Chemicals

ChemComp-MG:
MAGNESIUM ION / Magnesium

ChemComp-HOH:
WATER / Water

Source
  • lachnospiraceae bacterium (bacteria)
  • moraxella bovoculi (bacteria)
  • synthetic construct (others)
KeywordsBacterial Proteins / CRISPR-Associated Proteins / Cas12a protein / Cryoelectron Microscopy / DNA / Endodeoxyribonucleases / Enzyme Inhibitors / Gene Editing / Models, Molecular / Protein Binding / Protein Conformation / Protein Multimerization / RNA, Guide / RNA BINDING PROTEIN/RNA / CRISPR-Cas / anti-CRISPR / RNA BINDING PROTEIN / RNA BINDING PROTEIN-RNA complex

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