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TitleStructure of the Centromere Binding Factor 3 Complex from Kluyveromyces lactis.
Journal, issue, pagesJ Mol Biol, Vol. 431, Issue 22, Page 4444-4454, Year 2019
Publish dateNov 8, 2019
AuthorsPhong D Lee / Hui Wei / Dongyan Tan / Stephen C Harrison /
PubMed AbstractKinetochores are the multiprotein complexes that link chromosomal centromeres to mitotic-spindle microtubules. Budding yeast centromeres comprise three sequential "centromere-determining elements", ...Kinetochores are the multiprotein complexes that link chromosomal centromeres to mitotic-spindle microtubules. Budding yeast centromeres comprise three sequential "centromere-determining elements", CDEI, II, and III. CDEI (8 bp) and CDEIII (∼25 bp) are conserved between Kluyveromyces lactis and Saccharomyces cerevisiae, but CDEII in the former is twice as long (160 bp) as CDEII in the latter (80 bp). The CBF3 complex recognizes CDEIII and is required for assembly of a centromeric nucleosome, which in turn recruits other kinetochore components. To understand differences in centromeric nucleosome assembly between K. lactis and S. cerevisiae, we determined the structure of a K. lactis CBF3 complex by electron cryomicroscopy at ∼4 Å resolution and compared it with published structures of S. cerevisiae CBF3. We show differences in the pose of Ndc10 and discuss potential models of the K. lactis centromeric nucleosome that account for the extended CDEII length.
External linksJ Mol Biol / PubMed:31425683 / PubMed Central
MethodsEM (single particle)
Resolution4 - 4.3 Å
Structure data

EMDB-20270, PDB-6p7v:
Structure of the K. lactis CBF3 core
Method: EM (single particle) / Resolution: 4 Å

EMDB-20271, PDB-6p7w:
Structure of the K. lactis CBF3 core - Ndc10 D1 complex
Method: EM (single particle) / Resolution: 4.1 Å

EMDB-20272, PDB-6p7x:
Structure of the K. lactis CBF3 core - Ndc10 D1D2 complex
Method: EM (single particle) / Resolution: 4.3 Å

Source
  • kluyveromyces lactis (yeast)
KeywordsCentromere / Cryoelectron Microscopy / Fungal Proteins / Kinetochores / Kluyveromyces / Saccharomyces cerevisiae / Saccharomyces cerevisiae Proteins / DNA BINDING PROTEIN / yeast centromere-binding complex

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