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TitleCryo-EM Structures of a Group II Intron Reverse Splicing into DNA.
Journal, issue, pagesCell, Vol. 178, Issue 3, Page 612-623.e12, Year 2019
Publish dateJul 25, 2019
AuthorsDaniel B Haack / Xiaodong Yan / Cheng Zhang / Jason Hingey / Dmitry Lyumkis / Timothy S Baker / Navtej Toor /
PubMed AbstractGroup II introns are a class of retroelements that invade DNA through a copy-and-paste mechanism known as retrotransposition. Their coordinated activities occur within a complex that includes a ...Group II introns are a class of retroelements that invade DNA through a copy-and-paste mechanism known as retrotransposition. Their coordinated activities occur within a complex that includes a maturase protein, which promotes splicing through an unknown mechanism. The mechanism of splice site exchange within the RNA active site during catalysis also remains unclear. We determined two cryo-EM structures at 3.6-Å resolution of a group II intron reverse splicing into DNA. These structures reveal that the branch-site domain VI helix swings 90°, enabling substrate exchange during DNA integration. The maturase assists catalysis through a transient RNA-protein contact with domain VI that positions the branch-site adenosine for lariat formation during forward splicing. These findings provide the first direct evidence of the role the maturase plays during group II intron catalysis. The domain VI dynamics closely parallel spliceosomal branch-site helix movement and provide strong evidence for a retroelement origin of the spliceosome.
External linksCell / PubMed:31348888 / PubMed Central
MethodsEM (single particle)
Resolution3.6 Å
Structure data

9105

6me0

EMDB-9105, PDB-6me0:
Structure of a group II intron retroelement prior to DNA integration
Method: EM (single particle) / Resolution: 3.6 Å

9106

6mec

EMDB-9106, PDB-6mec:
Structure of a group II intron retroelement after DNA integration
Method: EM (single particle) / Resolution: 3.6 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-NA:
Unknown entry

Source
  • thermosynechococcus elongatus (bacteria)
  • thermosynechococcus elongatus (strain bp-1) (bacteria)
KeywordsRNA/DNA/Nucleic Acid Binding protein / Group II intron / Retroelement / Retrotransposition / RNA-DNA-Nucleic Acid Binding protein complex / RNA/DNA/RNA Binding Protein / RNA-DNA-RNA Binding Protein complex

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