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-Structure paper
タイトル | Structural insights into flagellar stator-rotor interactions. |
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ジャーナル・号・ページ | Elife, Vol. 8, Year 2019 |
掲載日 | 2019年7月17日 |
著者 | Yunjie Chang / Ki Hwan Moon / Xiaowei Zhao / Steven J Norris / Md A Motaleb / Jun Liu / |
PubMed 要旨 | The bacterial flagellar motor is a molecular machine that can rotate the flagellar filament at high speed. The rotation is generated by the stator-rotor interaction, coupled with an ion flux through ...The bacterial flagellar motor is a molecular machine that can rotate the flagellar filament at high speed. The rotation is generated by the stator-rotor interaction, coupled with an ion flux through the torque-generating stator. Here we employed cryo-electron tomography to visualize the intact flagellar motor in the Lyme disease spirochete, . By analyzing the motor structures of wild-type and stator-deletion mutants, we not only localized the stator complex in situ, but also revealed the stator-rotor interaction at an unprecedented detail. Importantly, the stator-rotor interaction induces a conformational change in the flagella C-ring. Given our observation that a non-motile mutant, in which proton flux is blocked, cannot generate the similar conformational change, we propose that the proton-driven torque is responsible for the conformational change required for flagellar rotation. |
リンク | Elife / PubMed:31313986 / PubMed Central |
手法 | EM (サブトモグラム平均) |
解像度 | 20.0 - 34.0 Å |
構造データ | EMDB-0534: EMDB-0536: EMDB-0537: EMDB-0538: |
由来 |
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