Gregor L Weiss / Ann-Katrin Kieninger / Iris Maldener / Karl Forchhammer / Martin Pilhofer /
PubMed Abstract
Multicellular lifestyle requires cell-cell connections. In multicellular cyanobacteria, septal junctions enable molecular exchange between sister cells and are required for cellular differentiation. ...Multicellular lifestyle requires cell-cell connections. In multicellular cyanobacteria, septal junctions enable molecular exchange between sister cells and are required for cellular differentiation. The structure of septal junctions is poorly understood, and it is unknown whether they are capable of controlling intercellular communication. Here, we resolved the in situ architecture of septal junctions by electron cryotomography of cryo-focused ion beam-milled cyanobacterial filaments. Septal junctions consisted of a tube traversing the septal peptidoglycan. Each tube end comprised a FraD-containing plug, which was covered by a cytoplasmic cap. Fluorescence recovery after photobleaching showed that intercellular communication was blocked upon stress. Gating was accompanied by a reversible conformational change of the septal junction cap. We provide the mechanistic framework for a cell junction that predates eukaryotic gap junctions by a billion years. The conservation of a gated dynamic mechanism across different domains of life emphasizes the importance of controlling molecular exchange in multicellular organisms.
EMDB-4949: Electron cryotomogram of septal region from FIB milled Anabaena sp. PCC7120 wild type filament Method: EM (tomography)
EMDB-4950: Electron cryotomogram of septal region from FIB milled Anabaena sp. PCC7120 gfp-fraD expressing filament Method: EM (tomography)
EMDB-4951: Electron cryotomogram of septal region from FIB milled Anabaena sp. PCC7120 SjcF1 mutant filament Method: EM (tomography)
EMDB-4952: Electron cryotomogram of septal region from FIB milled Anabaena sp. PCC7120 SepJ mutant filament Method: EM (tomography)
EMDB-4953: Electron cryotomogram of septal region from FIB milled Anabaena sp. PCC7120 FraD mutant filament Method: EM (tomography)
EMDB-4954: Electron cryotomogram of septal region from FIB milled Anabaena sp. PCC7120 FraC/FraD mutant filament Method: EM (tomography)
EMDB-4955: Electron cryotomogram of septal region from FIB milled Anabaena sp. PCC7120 FraC mutant filament Method: EM (tomography)
EMDB-4956: Electron cryotomogram of septal region from FIB milled Anabaena sp. PCC7120 AmiC1 mutant filament Method: EM (tomography)
EMDB-4957: Electron cryotomogram of septal region from FIB milled Anabaena sp. PCC7120 wild type filament after CCCP treatment Method: EM (tomography)
EMDB-4961: Subtomogram average of septal junctions from Anabaena sp. PCC7120 after 45s CCCP treatment Method: EM (subtomogram averaging) / Resolution: 35.0 Å
EMDB-4962: Subtomogram average of septal junctions from Anabaena sp. PCC7120 in the closed state after CCCP treatment Method: EM (subtomogram averaging) / Resolution: 28.0 Å
EMDB-4963: Subtomogram average of septal junctions from Anabaena sp. PCC7120 in the open state after washing out CCCP Method: EM (subtomogram averaging) / Resolution: 35.0 Å
EMDB-4964: Subtomogram average of septal junctions from Anabaena sp. PCC7120 AmiC1 mutant Method: EM (subtomogram averaging) / Resolution: 35.0 Å
EMDB-4965: Subtomogram average of septal junctions from Anabaena sp. PCC7120 SepJ mutant Method: EM (subtomogram averaging) / Resolution: 35.0 Å
EMDB-4966: Subtomogram average of septal junctions from Anabaena sp. PCC7120 SjcF1 mutant Method: EM (subtomogram averaging) / Resolution: 35.0 Å
EMDB-4967: Subtomogram average of septal junctions from Anabaena sp. PCC7120 gfp-fraD expressing mutant Method: EM (subtomogram averaging) / Resolution: 28.0 Å
EMDB-4968: Subtomogram average of septal junctions from Anabaena sp. PCC7120 after 24h in the dark Method: EM (subtomogram averaging) / Resolution: 35.0 Å
EMDB-4969: Subtomogram average of septal junctions from Anabaena sp. PCC7120 in the open state Method: EM (subtomogram averaging) / Resolution: 25.0 Å
Source
Nostoc sp. PCC 7120 (bacteria)
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Nostoc sp. PCC 7120 (bacteria)