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TitleStructural basis for transcription antitermination at bacterial intrinsic terminator.
Journal, issue, pagesNat Commun, Vol. 10, Issue 1, Page 3048, Year 2019
Publish dateJul 11, 2019
AuthorsLinlin You / Jing Shi / Liqiang Shen / Lingting Li / Chengli Fang / Chengzhi Yu / Wenbo Cheng / Yu Feng / Yu Zhang /
PubMed AbstractBacteriophages typically hijack the host bacterial transcriptional machinery to regulate their own gene expression and that of the host bacteria. The structural basis for bacteriophage protein- ...Bacteriophages typically hijack the host bacterial transcriptional machinery to regulate their own gene expression and that of the host bacteria. The structural basis for bacteriophage protein-mediated transcription regulation-in particular transcription antitermination-is largely unknown. Here we report the 3.4 Å and 4.0 Å cryo-EM structures of two bacterial transcription elongation complexes (P7-NusA-TEC and P7-TEC) comprising the bacteriophage protein P7, a master host-transcription regulator encoded by bacteriophage Xp10 of the rice pathogen Xanthomonas oryzae pv. Oryzae (Xoo) and discuss the mechanisms by which P7 modulates the host bacterial RNAP. The structures together with biochemical evidence demonstrate that P7 prevents transcription termination by plugging up the RNAP RNA-exit channel and impeding RNA-hairpin formation at the intrinsic terminator. Moreover, P7 inhibits transcription initiation by restraining RNAP-clamp motions. Our study reveals the structural basis for transcription antitermination by phage proteins and provides insights into bacterial transcription regulation.
External linksNat Commun / PubMed:31296855 / PubMed Central
MethodsEM (single particle)
Resolution3.41 - 3.95 Å
Structure data

9785

6j9e

EMDB-9785, PDB-6j9e:
Cryo-EM structure of Xanthomonos oryzae transcription elongation complex with NusA and the bacteriophage protein P7
Method: EM (single particle) / Resolution: 3.41 Å

9786

6j9f

EMDB-9786, PDB-6j9f:
Cryo-EM structure of Xanthomonos oryzae transcription elongation complex with the bacteriophage protein P7
Method: EM (single particle) / Resolution: 3.95 Å

Chemicals

ChemComp-MG:
Unknown entry

ChemComp-ZN:
Unknown entry

Source
  • xanthomonas oryzae pv. oryzae (bacteria)
  • xanthomonas oryzae pv. oryzae (strain pxo99a) (bacteria)
  • xanthomonas oryzae pv. oryzae pxo99a (bacteria)
  • xanthomonas virus xp10
  • synthetic construct (others)
  • Xanthomonas oryzae (bacteria)
  • xanthomonas oryzae pv. oryzae maff 311018 (bacteria)
KeywordsTRANSCRIPTION / RNA polymerase / transcription termination / anti-termination / RNAP clamp / phage / transcription initiation / P7 / NusA / Xanthomonos oryzae / Xp10

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