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TitleCryo-EM structure of the mammalian ATP synthase tetramer bound with inhibitory protein IF1.
Journal, issue, pagesScience, Vol. 364, Issue 6445, Page 1068-1075, Year 2019
Publish dateJun 14, 2019
AuthorsJinke Gu / Laixing Zhang / Shuai Zong / Runyu Guo / Tianya Liu / Jingbo Yi / Peiyi Wang / Wei Zhuo / Maojun Yang /
PubMed AbstractThe mitochondrial adenosine triphosphate (ATP) synthase produces most of the ATP required by mammalian cells. We isolated porcine tetrameric ATP synthase and solved its structure at 6.2-angstrom ...The mitochondrial adenosine triphosphate (ATP) synthase produces most of the ATP required by mammalian cells. We isolated porcine tetrameric ATP synthase and solved its structure at 6.2-angstrom resolution using a single-particle cryo-electron microscopy method. Two classical V-shaped ATP synthase dimers lie antiparallel to each other to form an H-shaped ATP synthase tetramer, as viewed from the matrix. ATP synthase inhibitory factor subunit 1 (IF1) is a well-known in vivo inhibitor of mammalian ATP synthase at low pH. Two IF1 dimers link two ATP synthase dimers, which is consistent with the ATP synthase tetramer adopting an inhibited state. Within the tetramer, we refined structures of intact ATP synthase in two different rotational conformations at 3.34- and 3.45-Å resolution.
External linksScience / PubMed:31197009
MethodsEM (single particle)
Resolution3.34 - 6.2 Å
Structure data

EMDB-0667, PDB-6j5k:
Cryo-EM structure of the mammalian ATP synthase tetramer bound with inhibitory protein IF1
Method: EM (single particle) / Resolution: 6.2 Å

EMDB-0668, PDB-6j54:
Cryo-EM structure of the mammalian E-state ATP synthase FO section
Method: EM (single particle) / Resolution: 3.9 Å

EMDB-0669, PDB-6j5j:
Cryo-EM structure of the mammalian E-state ATP synthase
Method: EM (single particle) / Resolution: 3.45 Å

EMDB-0670, PDB-6j5a:
Cryo-EM structure of the mammalian DP-state ATP synthase FO section
Method: EM (single particle) / Resolution: 4.35 Å

EMDB-0677, PDB-6j5i:
Cryo-EM structure of the mammalian DP-state ATP synthase
Method: EM (single particle) / Resolution: 3.34 Å

Chemicals

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM / Adenosine triphosphate

ChemComp-MG:
Unknown entry

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM / Adenosine diphosphate

Source
  • sus scrofa (pig)
  • Sus (mammal)
KeywordsMEMBRANE PROTEIN

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