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TitleStructural mechanism for regulation of the AAA-ATPases RUVBL1-RUVBL2 in the R2TP co-chaperone revealed by cryo-EM.
Journal, issue, pagesSci Adv, Vol. 5, Issue 5, Page eaaw1616, Year 2019
Publish dateMay 1, 2019
AuthorsHugo Muñoz-Hernández / Mohinder Pal / Carlos F Rodríguez / Rafael Fernandez-Leiro / Chrisostomos Prodromou / Laurence H Pearl / Oscar Llorca /
PubMed AbstractThe human R2TP complex (RUVBL1-RUVBL2-RPAP3-PIH1D1) is an HSP90 co-chaperone required for the maturation of several essential multiprotein complexes, including RNA polymerase II, small nucleolar ...The human R2TP complex (RUVBL1-RUVBL2-RPAP3-PIH1D1) is an HSP90 co-chaperone required for the maturation of several essential multiprotein complexes, including RNA polymerase II, small nucleolar ribonucleoproteins, and PIKK complexes such as mTORC1 and ATR-ATRIP. RUVBL1-RUVBL2 AAA-ATPases are also primary components of other essential complexes such as INO80 and Tip60 remodelers. Despite recent efforts, the molecular mechanisms regulating RUVBL1-RUVBL2 in these complexes remain elusive. Here, we report cryo-EM structures of R2TP and show how access to the nucleotide-binding site of RUVBL2 is coupled to binding of the client recruitment component of R2TP (PIH1D1) to its DII domain. This interaction induces conformational rearrangements that lead to the destabilization of an N-terminal segment of RUVBL2 that acts as a gatekeeper to nucleotide exchange. This mechanism couples protein-induced motions of the DII domains with accessibility of the nucleotide-binding site in RUVBL1-RUVBL2, and it is likely a general mechanism shared with other RUVBL1-RUVBL2-containing complexes.
External linksSci Adv / PubMed:31049401 / PubMed Central
MethodsEM (single particle)
Resolution3.75 - 5.15 Å
Structure data

4552

6qi8

EMDB-4552, PDB-6qi8:
Truncated human R2TP complex, structure 3 (ADP-filled)
Method: EM (single particle) / Resolution: 3.75 Å

4553

6qi9

EMDB-4553, PDB-6qi9:
Truncated human R2TP complex, structure 4 (ADP-empty)
Method: EM (single particle) / Resolution: 4.63 Å

EMDB-4554:
Truncated human R2TP complex, structure 1
Method: EM (single particle) / Resolution: 3.97 Å

EMDB-4555:
Truncated human R2TP complex, structure 2
Method: EM (single particle) / Resolution: 3.97 Å

EMDB-4556:
Truncated human R2TP complex, structure 5 (ADP-filled)
Method: EM (single particle) / Resolution: 3.97 Å

EMDB-4557:
Truncated human R2TP complex, structure 6 (ADP-empty)
Method: EM (single particle) / Resolution: 5.15 Å

Chemicals

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM / Adenosine diphosphate

Source
  • homo sapiens (human)
KeywordsCHAPERONE / R2TP / HSP90 co-chaperone / PIH1D1 / RPAP3 / RUVBL1 / RUVBL2 / cryo-EM

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