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TitleCryo-EM-On-a-Chip: Custom-Designed Substrates for the 3D Analysis of Macromolecules.
Journal, issue, pagesSmall, Vol. 15, Issue 21, Page e1900918, Year 2019
Publish dateApr 8, 2019
AuthorsNick A Alden / A Cameron Varano / William J Dearnaley / Maria J Solares / William Y Luqiu / Yanping Liang / Zhi Sheng / Sarah M McDonald / John Damiano / Jennifer McConnell / Madeline J Dukes / Deborah F Kelly /
PubMed AbstractThe fight against human disease requires a multidisciplinary scientific approach. Applying tools from seemingly unrelated areas, such as materials science and molecular biology, researchers can ...The fight against human disease requires a multidisciplinary scientific approach. Applying tools from seemingly unrelated areas, such as materials science and molecular biology, researchers can overcome long-standing challenges to improve knowledge of molecular pathologies. Here, custom-designed substrates composed of silicon nitride (SiN) are used to study the 3D attributes of tumor suppressor proteins that function in DNA repair events. New on-chip preparation strategies enable the isolation of native protein complexes from human cancer cells. Combined techniques of cryo-electron microscopy (EM) and molecular modeling reveal a new modified form of the p53 tumor suppressor present in aggressive glioblastoma multiforme cancer cells. Taken together, the findings provide a radical new design for cryo-EM substrates to evaluate the structures of disease-related macromolecules.
External linksSmall / PubMed:30963664 / PubMed Central
MethodsEM (single particle)
Resolution10.0 Å
Structure data

EMDB-0378:
p53 dimer assembly
Method: EM (single particle) / Resolution: 10.0 Å

Source
  • Homo sapiens (human)
  • Human (human)

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