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TitleHigh-resolution archaellum structure reveals a conserved metal-binding site.
Journal, issue, pagesEMBO Rep, Vol. 20, Issue 5, Year 2019
Publish dateMar 21, 2019
AuthorsVladimir A Meshcheryakov / Satoshi Shibata / Makoto Tokoro Schreiber / Alejandro Villar-Briones / Kenneth F Jarrell / Shin-Ichi Aizawa / Matthias Wolf /
PubMed AbstractMany archaea swim by means of archaella. While the archaellum is similar in function to its bacterial counterpart, its structure, composition, and evolution are fundamentally different. Archaella are ...Many archaea swim by means of archaella. While the archaellum is similar in function to its bacterial counterpart, its structure, composition, and evolution are fundamentally different. Archaella are related to archaeal and bacterial type IV pili. Despite recent advances, our understanding of molecular processes governing archaellum assembly and stability is still incomplete. Here, we determine the structures of archaella by X-ray crystallography and cryo-EM The crystal structure of FlaB1 is the first and only crystal structure of any archaellin to date at a resolution of 1.5 Å, which is put into biological context by a cryo-EM reconstruction from archaella at 4 Å resolution created with helical single-particle analysis. Our results indicate that the archaellum is predominantly composed of FlaB1. We identify N-linked glycosylation by cryo-EM and mass spectrometry. The crystal structure reveals a highly conserved metal-binding site, which is validated by mass spectrometry and electron energy-loss spectroscopy. We show that the metal-binding site, which appears to be a widespread property of archaellin, is required for filament integrity.
External linksEMBO Rep / PubMed:30898768 / PubMed Central
MethodsEM (helical sym.) / X-ray diffraction
Resolution1.5 - 4.0 Å
Structure data

6876

5z1l

EMDB-6876, PDB-5z1l:
Cryo-EM structure of Methanoccus maripaludis archaellum
Method: EM (helical sym.) / Resolution: 4.0 Å

PDB-5ya6:
Crystal structure of archaeal flagellin FlaB1 from Methanocaldococcus jannaschii
Method: X-RAY DIFFRACTION / Resolution: 1.5 Å

Chemicals

ChemComp-CA:
Unknown entry

ChemComp-HOH:
WATER / Water

Source
  • Methanococcus maripaludis S2 (archaea)
  • methanococcus maripaludis (strain s2 / ll) (archaea)
  • methanocaldococcus jannaschii dsm 2661 (archaea)
KeywordsSTRUCTURAL PROTEIN / archaea / flagellum / flagellin / PROTEIN FIBRIL / Archaellum / archea / metal binding / motility / helical

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