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TitleHomochiral and racemic MicroED structures of a peptide repeat from the ice-nucleation protein InaZ.
Journal, issue, pagesIUCrJ, Vol. 6, Issue Pt 2, Page 197-205, Year 2019
Publish dateMar 1, 2019
AuthorsChih-Te Zee / Calina Glynn / Marcus Gallagher-Jones / Jennifer Miao / Carlos G Santiago / Duilio Cascio / Tamir Gonen / Michael R Sawaya / Jose A Rodriguez /
PubMed AbstractThe ice-nucleation protein InaZ from contains a large number of degenerate repeats that span more than a quarter of its sequence and include the segment GSTSTA. structures of this repeat segment, ...The ice-nucleation protein InaZ from contains a large number of degenerate repeats that span more than a quarter of its sequence and include the segment GSTSTA. structures of this repeat segment, resolved to 1.1 Å by microfocus X-ray crystallography and to 0.9 Å by the cryo-EM method MicroED, were determined from both racemic and homochiral crystals. The benefits of racemic protein crystals for structure determination by MicroED were evaluated and it was confirmed that the phase restriction introduced by crystal centrosymmetry increases the number of successful trials during the phasing of the electron diffraction data. Both homochiral and racemic GSTSTA form amyloid-like protofibrils with labile, corrugated antiparallel β-sheets that mate face to back. The racemic GSTSTA protofibril represents a new class of amyloid assembly in which all-left-handed sheets mate with their all-right-handed counterparts. This determination of racemic amyloid assemblies by MicroED reveals complex amyloid architectures and illustrates the racemic advantage in macromolecular crystallography, now with submicrometre-sized crystals.
External linksIUCrJ / PubMed:30867917 / PubMed Central
MethodsX-ray diffraction / EM (electron crystallography)
Resolution0.9 - 1.101 Å
Structure data

PDB-6eex:
L-GSTSTA from degenerate octameric repeats in InaZ, residues 707-712
Method: X-RAY DIFFRACTION / Resolution: 1.1 Å

PDB-6m7m:
rac-GSTSTA from degenerate octameric repeats in InaZ, residues 707-712
Method: X-RAY DIFFRACTION / Resolution: 1.101 Å

PDB-6m9i:
L-GSTSTA from degenerate octameric repeats in InaZ, residues 707-712
Method: ELECTRON CRYSTALLOGRAPHY / Resolution: 0.9 Å

PDB-6m9j:
Racemic-GSTSTA from degenerate octameric repeats in InaZ, residues 707-712
Method: ELECTRON CRYSTALLOGRAPHY / Resolution: 0.9 Å

Chemicals

ChemComp-HOH:
WATER

Source
  • pseudomonas syringae (bacteria)
  • pseudomonas syringae pv. syringae (bacteria)
KeywordsPROTEIN FIBRIL / amyloid / racemic / ice nucleation / MicroED / InaZ / pseudomonas syringae

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