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-Structure paper
タイトル | CryoEM structure of adenovirus type 3 fibre with desmoglein 2 shows an unusual mode of receptor engagement. |
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ジャーナル・号・ページ | Nat Commun, Vol. 10, Issue 1, Page 1181, Year 2019 |
掲載日 | 2019年3月12日 |
著者 | Emilie Vassal-Stermann / Gregory Effantin / Chloe Zubieta / Wim Burmeister / Frédéric Iseni / Hongjie Wang / André Lieber / Guy Schoehn / Pascal Fender / |
PubMed 要旨 | Attachment of human adenovirus (HAd) to the host cell is a critical step of infection. Initial attachment occurs via the adenoviral fibre knob protein and a cellular receptor. Here we report the cryo- ...Attachment of human adenovirus (HAd) to the host cell is a critical step of infection. Initial attachment occurs via the adenoviral fibre knob protein and a cellular receptor. Here we report the cryo-electron microscopy (cryo-EM) structure of a <100 kDa non-symmetrical complex comprising the trimeric HAd type 3 fibre knob (HAd3K) and human desmoglein 2 (DSG2). The structure reveals a unique stoichiometry of 1:1 and 2:1 (DSG2: knob trimer) not previously observed for other HAd-receptor complexes. We demonstrate that mutating Asp261 in the fibre knob is sufficient to totally abolish receptor binding. These data shed new light on adenovirus infection strategies and provide insights for adenoviral vector development and structure-based design. |
リンク | Nat Commun / PubMed:30862836 / PubMed Central |
手法 | EM (単粒子) |
解像度 | 3.5 - 3.8 Å |
構造データ | |
由来 |
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キーワード | VIRAL PROTEIN / virus receptor / adenovirus / vector |