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TitleArchitectural principles for Hfq/Crc-mediated regulation of gene expression.
Journal, issue, pagesElife, Vol. 8, Year 2019
Publish dateFeb 13, 2019
AuthorsXue Yuan Pei / Tom Dendooven / Elisabeth Sonnleitner / Shaoxia Chen / Udo Bläsi / Ben F Luisi /
PubMed AbstractIn diverse bacterial species, the global regulator Hfq contributes to post-transcriptional networks that control expression of numerous genes. Hfq of the opportunistic pathogen inhibits translation ...In diverse bacterial species, the global regulator Hfq contributes to post-transcriptional networks that control expression of numerous genes. Hfq of the opportunistic pathogen inhibits translation of target transcripts by forming a regulatory complex with the catabolite repression protein Crc. This repressive complex acts as part of an intricate mechanism of preferred nutrient utilisation. We describe high-resolution cryo-EM structures of the assembly of Hfq and Crc bound to the translation initiation site of a target mRNA. The core of the assembly is formed through interactions of two cognate RNAs, two Hfq hexamers and a Crc pair. Additional Crc protomers are recruited to the core to generate higher-order assemblies with demonstrated regulatory activity in vivo. This study reveals how Hfq cooperates with a partner protein to regulate translation, and provides a structural basis for an RNA code that guides global regulators to interact cooperatively and regulate different RNA targets.
External linksElife / PubMed:30758287 / PubMed Central
MethodsEM (single particle)
Resolution3.13 - 3.37 Å
Structure data

0590

6o1k

EMDB-0590: Architectural principles for Hfq/Crc-mediated regulation of gene expression Hfq-Crc-amiE 2:2:2 complex (core complex)
PDB-6o1k: Architectural principles for Hfq/Crc-mediated regulation of gene expression. Hfq-Crc-amiE 2:2:2 complex (core complex)
Method: EM (single particle) / Resolution: 3.13 Å

0591

6o1l

EMDB-0591, PDB-6o1l:
Architectural principles for Hfq/Crc-mediated regulation of gene expression Hfq-Crc-amiE 2:3:2 complex
Method: EM (single particle) / Resolution: 3.37 Å

0592

6o1m

EMDB-0592: Architectural principles for Hfq/Crc-mediated regulation of gene expression Hfq-Crc-amiE 2:4:2 complex
PDB-6o1m: Architectural principles for Hfq/Crc-mediated regulation of gene expression. Hfq-Crc-amiE 2:4:2 complex
Method: EM (single particle) / Resolution: 3.15 Å

Source
  • pseudomonas aeruginosa (bacteria)
  • pseudomonas aeruginosa (strain atcc 15692 / dsm 22644 / cip 104116 / jcm 14847 / lmg 12228 / 1c / prs 101 / pao1) (bacteria)
KeywordsRNA BINDING PROTEIN/RNA/Hydrolase / Hfq / Crc / amiE / Carbon catabolite repression / RNA-protein interaction / RNA-mediated gene regulation / RNA BINDING PROTEIN / RNA BINDING PROTEIN-RNA-Hydrolase complex

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