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-Structure paper
タイトル | Enhancement of tomogram interpretability using the locked self-rotation function. |
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ジャーナル・号・ページ | J Struct Biol, Vol. 205, Issue 3, Page 53-58, Year 2019 |
掲載日 | 2019年3月1日 |
著者 | Andrei Fokine / Baldeep Khare / Yingyuan Sun / Michael G Rossmann / |
PubMed 要旨 | The interpretation of cryo-electron tomograms of macromolecular complexes can be difficult because of the large amount of noise and because of the missing wedge effect. Here it is shown how the ...The interpretation of cryo-electron tomograms of macromolecular complexes can be difficult because of the large amount of noise and because of the missing wedge effect. Here it is shown how the presence of rotational symmetry in a sample can be utilized to enhance the quality of a tomographic analysis. The orientation of symmetry axes in a sub-tomogram can be determined using a locked self-rotation function. Given this knowledge, the sub-tomogram density can then be averaged to improve its interpretability. Sub-tomograms of the icosahedral bacteriophage phiX174 are used to demonstrate the procedure. |
リンク | J Struct Biol / PubMed:30742896 / PubMed Central |
手法 | EM (トモグラフィー) |
構造データ | EMDB-9040: |