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TitleThermostable Branched-Chain Amino Acid Transaminases From the Archaea Geoglobus acetivorans and Archaeoglobus fulgidus : Biochemical and Structural Characterization.
Journal, issue, pagesFront Bioeng Biotechnol, Vol. 7, Page 7-7, Year 2019
Publish dateJul 16, 2015 (structure data deposition date)
AuthorsIsupov, M.N. / Boyko, K.M. / Sutter, J.M. / James, P. / Sayer, C. / Schmidt, M. / Schonheit, P. / Nikolaeva, A.Y. / Stekhanova, T.N. / Mardanov, A.V. ...Isupov, M.N. / Boyko, K.M. / Sutter, J.M. / James, P. / Sayer, C. / Schmidt, M. / Schonheit, P. / Nikolaeva, A.Y. / Stekhanova, T.N. / Mardanov, A.V. / Ravin, N.V. / Bezsudnova, E.Y. / Popov, V.O. / Littlechild, J.A.
External linksFront Bioeng Biotechnol / PubMed:30733943
MethodsX-ray diffraction
Resolution1.9 - 2.2 Å
Structure data

PDB-5cm0:
Crystal structure of branched-chain aminotransferase from thermophilic archaea Geoglobus acetivorans
Method: X-RAY DIFFRACTION / Resolution: 1.9 Å

PDB-5e25:
Crystal structure of branched-chain aminotransferase from thermophilic archaea Geoglobus acetivorans complexed with alpha-ketoglutarate
Method: X-RAY DIFFRACTION / Resolution: 2.2 Å

PDB-5mqz:
Archaeal branched-chain amino acid aminotransferase from Archaeoglobus fulgidus; holoform
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-5mr0:
Thermophilic archaeal branched-chain amino acid transaminases from Geoglobus acetivorans and Archaeoglobus fulgidus: biochemical and structural characterisation
Method: X-RAY DIFFRACTION / Resolution: 1.98 Å

Chemicals

ChemComp-PLP:
PYRIDOXAL-5'-PHOSPHATE

ChemComp-GOL:
GLYCEROL

ChemComp-HOH:
WATER

ChemComp-AKG:
2-OXOGLUTARIC ACID

ChemComp-CL:
Unknown entry

ChemComp-PEG:
DI(HYDROXYETHYL)ETHER

ChemComp-TAM:
TRIS(HYDROXYETHYL)AMINOMETHANE / pH buffer*YM

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-PXG:
3-[O-PHOSPHONOPYRIDOXYL]--AMINO-BENZOIC ACID

Source
  • geoglobus acetivorans (archaea)
  • archaeoglobus fulgidus (strain atcc 49558 / vc-16 / dsm 4304 / jcm 9628 / nbrc 100126) (archaea)
KeywordsTRANSFERASE / aminotransferase / branched-chain / PLP / BCAT / Geoglobus acetivorans / alpha-ketoglutarate / complex / branch-chain transaminase / thermophilic archaea / substrate specificity / branch-chain transaminases

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