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Structure paper

TitleArchitecture of Microcin B17 Synthetase: An Octameric Protein Complex Converting a Ribosomally Synthesized Peptide into a DNA Gyrase Poison.
Journal, issue, pagesMol. Cell, Vol. 73, Page 749-762.e5, Year 2019
Publish dateJun 4, 2018 (structure data deposition date)
AuthorsGhilarov, D. / Stevenson, C.E.M. / Travin, D.Y. / Piskunova, J. / Serebryakova, M. / Maxwell, A. / Lawson, D.M. / Severinov, K.
External linksMol. Cell / PubMed:30661981
MethodsX-ray diffraction
Resolution1.85 - 2.7 Å
Structure data

PDB-6gos:
E. coli Microcin synthetase McbBCD complex with pro-MccB17 bound
Method: X-RAY DIFFRACTION / Resolution: 2.1 Å

PDB-6grg:
E. coli Microcin synthetase McbBCD complex with pro-MccB17, ADP and phosphate bound
Method: X-RAY DIFFRACTION / Resolution: 2.35 Å

PDB-6grh:
E. coli Microcin synthetase McbBCD complex with truncated pro-MccB17 bound
Method: X-RAY DIFFRACTION / Resolution: 1.85 Å

PDB-6gri:
E. coli Microcin synthetase McbBCD complex
Method: X-RAY DIFFRACTION / Resolution: 2.7 Å

Chemicals

ChemComp-ZN:
Unknown entry

ChemComp-SO4:
SULFATE ION

ChemComp-CL:
Unknown entry

ChemComp-EDO:
1,2-ETHANEDIOL

ChemComp-FMN:
FLAVIN MONONUCLEOTIDE

ChemComp-GOL:
GLYCEROL

ChemComp-HOH:
WATER

ChemComp-ATP:
ADENOSINE-5'-TRIPHOSPHATE / ATP, energy-carrying molecule*YM

ChemComp-ADP:
ADENOSINE-5'-DIPHOSPHATE / ADP, energy-carrying molecule*YM

ChemComp-PO4:
PHOSPHATE ION

ChemComp-MG:
Unknown entry

Source
  • escherichia coli str. k-12 substr. mg1655 (bacteria)
  • escherichia coli (E. coli)
KeywordsAntibiotic/inhibitor / microcin / DNA gyrase / heterocyclization / posttranslational modification / BIOSYNTHETIC PROTEIN / Antibiotic-inhibitor complex

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